2j8f

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{{STRUCTURE_2j8f| PDB=2j8f | SCENE= }}
{{STRUCTURE_2j8f| PDB=2j8f | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE MODULAR CPL-1 ENDOLYSIN COMPLEXED WITH A PEPTIDOGLYCAN ANALOGUE (E94Q MUTANT IN COMPLEX WITH A DISACCHARIDE-PENTAPEPTIDE)'''
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===CRYSTAL STRUCTURE OF THE MODULAR CPL-1 ENDOLYSIN COMPLEXED WITH A PEPTIDOGLYCAN ANALOGUE (E94Q MUTANT IN COMPLEX WITH A DISACCHARIDE-PENTAPEPTIDE)===
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==Overview==
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Pneumococcal bacteriophage-encoded lysins are modular proteins that have been shown to act as enzymatic antimicrobial agents (enzybiotics) in treatment of streptococcal infections. The first x-ray crystal structures of the Cpl-1 lysin, encoded by the pneumococcal phage Cp-1, in complex with three bacterial cell wall peptidoglycan (PG) analogues are reported herein. The Cpl-1 structure is folded in two well defined modules, one responsible for anchoring to the pneumococcal cell wall and the other, a catalytic module, that hydrolyzes the PG. Conformational rearrangement of Tyr-127 is a critical event in molecular recognition of a stretch of five saccharide rings of the polymeric peptidoglycan (cell wall). The PG is bound at a stretch of the surface that is defined as the peptidoglycan-binding sites 1 and 2, the juncture of which catalysis takes place. The peptidoglycan-binding site 1 binds to a stretch of three saccharides of the peptidoglycan in a conformation essentially identical to that of the peptidoglycan in solution. In contrast, binding of two peptidoglycan saccharides at the peptidoglycan-binding site 2 introduces a kink into the solution structure of the peptidoglycan, en route to catalytic turnover. These findings provide the first structural evidence on recognition of the peptidoglycan and shed light on the discrete events of cell wall degradation by Cpl-1.
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{{ABSTRACT_PUBMED_17581815}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1., Perez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Paez JA, Garcia P, Martinez-Ripoll M, Garcia JL, Mobashery S, Menendez M, Hermoso JA, J Biol Chem. 2007 Aug 24;282(34):24990-9. Epub 2007 Jun 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17581815 17581815]
Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1., Perez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Paez JA, Garcia P, Martinez-Ripoll M, Garcia JL, Mobashery S, Menendez M, Hermoso JA, J Biol Chem. 2007 Aug 24;282(34):24990-9. Epub 2007 Jun 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17581815 17581815]
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Structural basis for selective recognition of pneumococcal cell wall by modular endolysin from phage Cp-1., Hermoso JA, Monterroso B, Albert A, Galan B, Ahrazem O, Garcia P, Martinez-Ripoll M, Garcia JL, Menendez M, Structure. 2003 Oct;11(10):1239-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14527392 14527392]
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Crystallization and preliminary X-ray diffraction studies of the complete modular endolysin from Cp-1, a phage infecting Streptococcus pneumoniae., Monterroso B, Albert A, Martinez-Ripoll M, Garcia P, Garcia JL, Menendez M, Hermoso JA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1487-9. Epub 2002, Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12198311 12198311]
[[Category: Bacteriophage cp-1]]
[[Category: Bacteriophage cp-1]]
[[Category: Lysozyme]]
[[Category: Lysozyme]]
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[[Category: Multimodular]]
[[Category: Multimodular]]
[[Category: Pneumococcal cell wall degradation]]
[[Category: Pneumococcal cell wall degradation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:30:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:20:47 2008''

Revision as of 17:20, 28 July 2008

Image:2j8f.png

Template:STRUCTURE 2j8f

CRYSTAL STRUCTURE OF THE MODULAR CPL-1 ENDOLYSIN COMPLEXED WITH A PEPTIDOGLYCAN ANALOGUE (E94Q MUTANT IN COMPLEX WITH A DISACCHARIDE-PENTAPEPTIDE)

Template:ABSTRACT PUBMED 17581815

About this Structure

2J8F is a Single protein structure of sequence from Bacteriophage cp-1. Full crystallographic information is available from OCA.

Reference

Elucidation of the molecular recognition of bacterial cell wall by modular pneumococcal phage endolysin CPL-1., Perez-Dorado I, Campillo NE, Monterroso B, Hesek D, Lee M, Paez JA, Garcia P, Martinez-Ripoll M, Garcia JL, Mobashery S, Menendez M, Hermoso JA, J Biol Chem. 2007 Aug 24;282(34):24990-9. Epub 2007 Jun 19. PMID:17581815

Structural basis for selective recognition of pneumococcal cell wall by modular endolysin from phage Cp-1., Hermoso JA, Monterroso B, Albert A, Galan B, Ahrazem O, Garcia P, Martinez-Ripoll M, Garcia JL, Menendez M, Structure. 2003 Oct;11(10):1239-49. PMID:14527392

Crystallization and preliminary X-ray diffraction studies of the complete modular endolysin from Cp-1, a phage infecting Streptococcus pneumoniae., Monterroso B, Albert A, Martinez-Ripoll M, Garcia P, Garcia JL, Menendez M, Hermoso JA, Acta Crystallogr D Biol Crystallogr. 2002 Sep;58(Pt 9):1487-9. Epub 2002, Aug 23. PMID:12198311

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