2pvi

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{{STRUCTURE_2pvi| PDB=2pvi | SCENE= }}
{{STRUCTURE_2pvi| PDB=2pvi | SCENE= }}
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'''PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA'''
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===PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA===
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==Overview==
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In restriction-modification systems, cleavage of substrate sites in cellular DNA by the restriction endonuclease is prevented by the action of a cognate methyltransferase that acts on the same substrate sites. The PvuII restriction endonuclease (R.PvuII) has been structurally characterized in a complex with substrate DNA (Cheng et al., 1994) and as an apoenzyme (Athanasiadis et al., 1994). We report here a structure, determined to 1.9 A resolution by crystallography, of a complex between R.PvuII and iodinated DNA. The presence of an iodine at the 5-carbon of the methylatable cytosine results in the following changes in the protein: His84 moved away from the modified base; this movement was amplified in His85 and disrupts an intersubunit hydrogen bond; and the base modification disturbs the distribution of water molecules that associate with these histidine residues and the area of the scissile bond. Considering these observations, hypotheses are given as to why a similar oligonucleotide, where a methyl group resides on the 5-carbon of the methylatable cytosine, is slowly cleaved by R.PvuII (Rice et al., 1995).
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(as it appears on PubMed at http://www.pubmed.gov), where 9628337 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9628337}}
==About this Structure==
==About this Structure==
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[[Category: Cheng, X.]]
[[Category: Cheng, X.]]
[[Category: Horton, J.]]
[[Category: Horton, J.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 13:52:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:25:19 2008''

Revision as of 17:25, 28 July 2008

Image:2pvi.png

Template:STRUCTURE 2pvi

PVUII ENDONUCLEASE COMPLEXED TO AN IODINATED COGNATE DNA

Template:ABSTRACT PUBMED 9628337

About this Structure

2PVI is a Single protein structure of sequence from Proteus vulgaris. Full crystallographic information is available from OCA.

Reference

How is modification of the DNA substrate recognized by the PvuII restriction endonuclease?, Horton JR, Bonventre J, Cheng X, Biol Chem. 1998 Apr-May;379(4-5):451-8. PMID:9628337

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