2d6o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2d6o.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2d6o.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2d6o| PDB=2d6o | SCENE= }}
{{STRUCTURE_2d6o| PDB=2d6o | SCENE= }}
-
'''Crystal structure of mouse galectin-9 N-terminal CRD in complex with N-acetyllactosamine dimer'''
+
===Crystal structure of mouse galectin-9 N-terminal CRD in complex with N-acetyllactosamine dimer===
-
==Overview==
+
<!--
-
The galectins are a family of beta-galactoside-binding animal lectins with a conserved carbohydrate recognition domain (CRD). They have a high affinity for small beta-galactosides, but binding specificity for complex glycoconjugates varies considerably within the family. The ligand recognition is essential for their proper function, and the structures of several galectins have suggested their mechanism of carbohydrate binding. Galectin-9 has two tandem CRDs with a short linker, and we report the crystal structures of mouse galectin-9 N-terminal CRD (NCRD) in the absence and the presence of four ligand complexes. All structures form the same dimer, which is quite different from the canonical 2-fold symmetric dimer seen for galectin-1 and -2. The beta-galactoside recognition mechanism in the galectin-9 NCRD is highly conserved among other galectins. In the apo form structure, water molecules mimic the ligand hydrogen-bond network. The galectin-9 NCRD can bind both N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc) with the proper location of Arg-64. Moreover, the structure of the N-acetyllactosamine dimer (Galbeta1-4GlcNAcbeta1-3Galbeta1-4GlcNAc) complex shows a unique binding mode of galectin-9. Finally, surface plasmon resonance assay showed that the galectin-9 NCRD forms a homophilic dimer not only in the crystal but also in solution.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16990264}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16990264 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16990264}}
==About this Structure==
==About this Structure==
Line 31: Line 35:
[[Category: Carbohydrate binding protein]]
[[Category: Carbohydrate binding protein]]
[[Category: Galectin]]
[[Category: Galectin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:47:34 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:34:30 2008''

Revision as of 17:34, 28 July 2008

Image:2d6o.png

Template:STRUCTURE 2d6o

Crystal structure of mouse galectin-9 N-terminal CRD in complex with N-acetyllactosamine dimer

Template:ABSTRACT PUBMED 16990264

About this Structure

2D6O is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the galectin-9 N-terminal carbohydrate recognition domain from Mus musculus reveals the basic mechanism of carbohydrate recognition., Nagae M, Nishi N, Murata T, Usui T, Nakamura T, Wakatsuki S, Kato R, J Biol Chem. 2006 Nov 24;281(47):35884-93. Epub 2006 Sep 21. PMID:16990264

Page seeded by OCA on Mon Jul 28 20:34:30 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2d6o"
Personal tools