This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1t41

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1t41.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1t41.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1t41| PDB=1t41 | SCENE= }}
{{STRUCTURE_1t41| PDB=1t41 | SCENE= }}
-
'''Crystal structure of human aldose reductase complexed with NADP and IDD552'''
+
===Crystal structure of human aldose reductase complexed with NADP and IDD552===
-
==Overview==
+
<!--
-
The X-ray crystal structure of human aldose reductase (ALR2) in complex with the inhibitor IDD552 was determined using crystals obtained from two crystallization conditions with different pH values (pH 5 and 8). In both structures the charged carboxylic head of the inhibitor binds to the active site, making hydrogen-bond interactions with His110 and Tyr48 and electrostatic interactions with NADP+. There is an important difference between the two structures: the observation of a double conformation of the carboxylic acid moiety of the inhibitor at pH 8, with one water molecule interacting with the main configuration. This is the first time that a water molecule has been observed deep inside the ALR2 active site. Furthermore, in the configuration with the lower occupancy factor the difference electron-density map shows a clear peak (2.5sigma) for the H atom in the hydrogen bond between the inhibitor's carboxylic acid and the Tyr48 side-chain O atom. The position of this peak implies that this H atom is shared between both O atoms, indicating possible direct proton transfer from this residue to the inhibitor. This fact agrees with the model of the catalytic mechanism, in which the proton is donated by the Tyr48 hydroxyl to the substrate. These observations are useful both in drug design and in understanding the ALR2 mechanism.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15272156}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15272156 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15272156}}
==About this Structure==
==About this Structure==
Line 36: Line 40:
[[Category: Ternary complex]]
[[Category: Ternary complex]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:29:54 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:35:10 2008''

Revision as of 17:35, 28 July 2008

Image:1t41.png

Template:STRUCTURE 1t41

Crystal structure of human aldose reductase complexed with NADP and IDD552

Template:ABSTRACT PUBMED 15272156

About this Structure

1T41 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystallographic structure of the aldose reductase-IDD552 complex shows direct proton donation from tyrosine 48., Ruiz F, Hazemann I, Mitschler A, Joachimiak A, Schneider T, Karplus M, Podjarny A, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1347-54. Epub 2004, Jul 21. PMID:15272156

Page seeded by OCA on Mon Jul 28 20:35:10 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t41"
Personal tools