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2ilz

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{{STRUCTURE_2ilz| PDB=2ilz | SCENE= }}
{{STRUCTURE_2ilz| PDB=2ilz | SCENE= }}
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'''Crystal structure of poliovirus polymerase complexed with GTP and Mn2+'''
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===Crystal structure of poliovirus polymerase complexed with GTP and Mn2+===
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==Overview==
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The viral RNA-dependent RNA polymerases show a conserved structure where the fingers domain interacts with the top of the thumb domain to create a tunnel through which nucleotide triphosphates reach the active site. We have solved the crystal structures of poliovirus polymerase (3D(pol)) in complex with all four NTPs, showing that they all bind in a common pre-insertion site where the phosphate groups are not yet positioned over the active site. The NTPs interact with both the fingers and palm domains, forming bridging interactions that explain the increased thermal stability of 3D(pol) in the presence of NTPs. We have also examined the importance of the fingers-thumb domain interaction for the function and structural stability of 3D(pol). Results from thermal denaturation experiments using circular dichroism and 2-anilino-6-napthaline-sulfonate (ANS) fluorescence show that 3D(pol) has a melting temperature of only approximately 40 degrees C. NTP binding stabilizes the protein and increases the melting by 5-6 degrees C while mutations in the fingers-thumb domain interface destabilize the protein and reduce the melting point by as much as 6 degrees C. In particular, the burial of Phe30 and Phe34 from the tip of the index finger into a pocket at the top of the thumb and the presence of Trp403 on the thumb domain are key interactions required to maintain the structural integrity of the polymerase. The data suggest the fingers domain has significant conformational flexibility and exists in a highly dynamic molten globule state at physiological temperature. The role of the enclosed active site motif as a structural scaffold for constraining the fingers domain and accommodating conformational changes in 3D(pol) and other viral polymerases during the catalytic cycle is discussed.
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{{ABSTRACT_PUBMED_17223130}}
==About this Structure==
==About this Structure==
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[[Category: Rna-dependent]]
[[Category: Rna-dependent]]
[[Category: Stabilization]]
[[Category: Stabilization]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:38:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:45:07 2008''

Revision as of 17:45, 28 July 2008

Image:2ilz.png

Template:STRUCTURE 2ilz

Crystal structure of poliovirus polymerase complexed with GTP and Mn2+

Template:ABSTRACT PUBMED 17223130

About this Structure

2ILZ is a Single protein structure of sequence from Human poliovirus 1. Full crystallographic information is available from OCA.

Reference

Stabilization of poliovirus polymerase by NTP binding and fingers-thumb interactions., Thompson AA, Albertini RA, Peersen OB, J Mol Biol. 2007 Mar 9;366(5):1459-74. Epub 2006 Dec 1. PMID:17223130

Page seeded by OCA on Mon Jul 28 20:45:07 2008

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