1xem

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{{STRUCTURE_1xem| PDB=1xem | SCENE= }}
{{STRUCTURE_1xem| PDB=1xem | SCENE= }}
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'''High Resolution Crystal Structure of Escherichia coli Zinc- Peptide Deformylase bound to formate'''
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===High Resolution Crystal Structure of Escherichia coli Zinc- Peptide Deformylase bound to formate===
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==Overview==
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E. coli peptide deformylase (PDF) catalyzes the deformylation of nascent polypeptides generated during protein synthesis. While PDF was originally thought to be a zinc enzyme, subsequent studies revealed that the active site metal is iron. In an attempt to understand this unusual metal preference, high-resolution structures of Fe-, Co-, and Zn-PDF were determined in complex with its deformylation product, formate. In all three structures, the formate ion binds the metal and forms hydrogen-bonding interactions with the backbone nitrogen of Leu91, the amide side chain of Gln50, and the carboxylate side chain of Glu133. One key difference, however, is how the formate binds the metal. In Fe-PDF and Co-PDF, formate binds in a bidentate fashion, while in Zn-PDF, it binds in a monodentate fashion. Importantly, these structural results provide the first clues into the origins of PDF's metal-dependent activity differences. On the basis of these structures, we propose that the basis for the higher activity of Fe-PDF stems from the better ability of iron to bind and activate the tetrahedral transition state required for cleavage of the N-terminal formyl group.
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(as it appears on PubMed at http://www.pubmed.gov), where 15796505 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15796505}}
==About this Structure==
==About this Structure==
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[[Category: Formate]]
[[Category: Formate]]
[[Category: Zinc deformylase]]
[[Category: Zinc deformylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:55:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 20:58:52 2008''

Revision as of 17:58, 28 July 2008

Image:1xem.png

Template:STRUCTURE 1xem

High Resolution Crystal Structure of Escherichia coli Zinc- Peptide Deformylase bound to formate

Template:ABSTRACT PUBMED 15796505

About this Structure

1XEM is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structures of E. coli peptide deformylase bound to formate: insight into the preference for Fe2+ over Zn2+ as the active site metal., Jain R, Hao B, Liu RP, Chan MK, J Am Chem Soc. 2005 Apr 6;127(13):4558-9. PMID:15796505

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