2qmo

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{{STRUCTURE_2qmo| PDB=2qmo | SCENE= }}
{{STRUCTURE_2qmo| PDB=2qmo | SCENE= }}
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'''Crystal structure of dethiobiotin synthetase (bioD) from Helicobacter pylori'''
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===Crystal structure of dethiobiotin synthetase (bioD) from Helicobacter pylori===
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==Overview==
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We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
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{{ABSTRACT_PUBMED_17982461}}
==About this Structure==
==About this Structure==
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[[Category: Psi-2]]
[[Category: Psi-2]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:13:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:06:03 2008''

Revision as of 18:06, 28 July 2008

Image:2qmo.png

Template:STRUCTURE 2qmo

Crystal structure of dethiobiotin synthetase (bioD) from Helicobacter pylori

Template:ABSTRACT PUBMED 17982461

About this Structure

2QMO is a Single protein structure of sequence from Helicobacter pylori 26695. Full crystallographic information is available from OCA.

Reference

In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461

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