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1ny7

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(New page: 200px<br /><applet load="1ny7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ny7, resolution 3.00&Aring;" /> '''COWPEA MOSAIC VIRUS ...)
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Revision as of 20:31, 20 November 2007


1ny7, resolution 3.00Å

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COWPEA MOSAIC VIRUS (CPMV)

Overview

Comoviruses are a group of plant viruses in the picornavirus superfamily., The type member of comoviruses, cowpea mosaic virus (CPMV), was, crystallized in the cubic space group I23, a = 317 A and the hexagonal, space group P6(1)22, a = 451 A, c = 1038 A. Structures of three closely, similar nucleoprotein particles were determined in the cubic form. The, roughly 300-A capsid was similar to the picornavirus capsid displaying a, pseudo T = 3 (P = 3) surface lattice. The three beta-sandwich domains, adopt two orientations, one with the long axis radial and the other two, with the long axes tangential in reference to the capsid sphere. T = 3, viruses display one or the other of these two orientations. The CPMV, capsid was permeable to cesium ions, leading to a disturbance of the, beta-annulus inside a channel-like structure, suggesting an ion channel., The hexagonal crystal form diffracted X rays to 3 A resolution, despite, the large unit cell. The large ( approximately 200 A) solvent channels in, the lattice allow exchange of CPMV cognate Fab fragments. As an initial, step in the structure determination of the CPMV/Fab complex, the P6(1)22, crystal structure was solved by molecular replacement with the CPMV model, determined in the cubic cell.

About this Structure

1NY7 is a Protein complex structure of sequences from Cowpea mosaic virus. Full crystallographic information is available from OCA.

Reference

The refined crystal structure of cowpea mosaic virus at 2.8 A resolution., Lin T, Chen Z, Usha R, Stauffacher CV, Dai JB, Schmidt T, Johnson JE, Virology. 1999 Dec 5;265(1):20-34. PMID:10603314

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