2p9i

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{{STRUCTURE_2p9i| PDB=2p9i | SCENE= }}
{{STRUCTURE_2p9i| PDB=2p9i | SCENE= }}
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'''Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde'''
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===Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde===
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==Overview==
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ATP is required for nucleation of actin filament branches by Arp2/3 complex, but the influence of ATP binding and hydrolysis are poorly understood. We determined crystal structures of bovine Arp2/3 complex cocrystallized with various bound adenine nucleotides and cations. Nucleotide binding favors closure of the nucleotide-binding cleft of Arp3, but no large-scale conformational changes in the complex. Thus, ATP binding does not directly activate Arp2/3 complex but is part of a network of interactions that contribute to nucleation. We compared nucleotide-induced conformational changes of residues lining the cleft in Arp3 and actin structures to construct a movie depicting the proposed ATPase cycle for the actin family. Chemical crosslinking stabilized subdomain 1 of Arp2, revealing new electron density for 69 residues in this subdomain. Steric clashes with Arp3 appear to be responsible for intrinsic disorder of subdomains 1 and 2 of Arp2 in inactive Arp2/3 complex.
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The line below this paragraph, {{ABSTRACT_PUBMED_17499050}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17499050 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17499050}}
==About this Structure==
==About this Structure==
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[[Category: Complex]]
[[Category: Complex]]
[[Category: Wd repeat]]
[[Category: Wd repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:40:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:14:39 2008''

Revision as of 18:14, 28 July 2008

Image:2p9i.png

Template:STRUCTURE 2p9i

Crystal Structure of bovine Arp2/3 Complex co-crystallized with ADP and crosslinked with gluteraldehyde

Template:ABSTRACT PUBMED 17499050

About this Structure

2P9I is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex., Nolen BJ, Pollard TD, Mol Cell. 2007 May 11;26(3):449-57. PMID:17499050

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