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1nt4

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{{STRUCTURE_1nt4| PDB=1nt4 | SCENE= }}
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'''Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate'''
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===Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate===
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==Overview==
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The Escherichia coli periplasmic glucose-1-phosphatase is a member of the histidine acid phosphatase family and acts primarily as a glucose scavenger. Previous substrate profiling studies have demonstrated some of the intriguing properties of the enzyme, including its unique and highly selective inositol phosphatase activity. The enzyme is also potentially involved in pathogenic inositol phosphate signal transduction pathways via type III secretion into the host cell. We have determined the crystal structure of E. coli glucose-1-phosphatase in an effort to unveil the structural mechanism underlying such unique substrate specificity. The structure was determined by the method of multiwavelength anomalous dispersion using a tungstate derivative together with the H18A inactive mutant complex structure with glucose 1-phosphate at 2.4-A resolution. In the active site of glucose-1-phosphatase, there are two unique gating residues, Glu-196 and Leu-24, in addition to the conserved features of histidine acid phosphatases. Together they create steric and electrostatic constraints responsible for the unique selectivity of the enzyme toward phytate and glucose-1-phosphate as well as its unusually high pH optimum for the latter. Based on the structural characterization, we were able to derive simple structural principles that not only precisely explains the substrate specificity of glucose-1-phosphatase and the hydrolysis products of various inositol phosphate substrates but also rationalizes similar general characteristics across the histidine acid phosphatase family.
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(as it appears on PubMed at http://www.pubmed.gov), where 12782623 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12782623}}
==About this Structure==
==About this Structure==
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Revision as of 18:23, 28 July 2008

Image:1nt4.png

Template:STRUCTURE 1nt4

Crystal structure of Escherichia coli periplasmic glucose-1-phosphatase H18A mutant complexed with glucose-1-phosphate

Template:ABSTRACT PUBMED 12782623

About this Structure

1NT4 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Functional insights revealed by the crystal structures of Escherichia coli glucose-1-phosphatase., Lee DC, Cottrill MA, Forsberg CW, Jia Z, J Biol Chem. 2003 Aug 15;278(33):31412-8. Epub 2003 Jun 1. PMID:12782623

Page seeded by OCA on Mon Jul 28 21:23:18 2008

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