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2rkj

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[[Image:2rkj.jpg|left|200px]]
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{{STRUCTURE_2rkj| PDB=2rkj | SCENE= }}
{{STRUCTURE_2rkj| PDB=2rkj | SCENE= }}
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'''Cocrystal structure of a tyrosyl-tRNA synthetase splicing factor with a group I intron RNA'''
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===Cocrystal structure of a tyrosyl-tRNA synthetase splicing factor with a group I intron RNA===
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==Overview==
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The 'RNA world' hypothesis holds that during evolution the structural and enzymatic functions initially served by RNA were assumed by proteins, leading to the latter's domination of biological catalysis. This progression can still be seen in modern biology, where ribozymes, such as the ribosome and RNase P, have evolved into protein-dependent RNA catalysts ('RNPzymes'). Similarly, group I introns use RNA-catalysed splicing reactions, but many function as RNPzymes bound to proteins that stabilize their catalytically active RNA structure. One such protein, the Neurospora crassa mitochondrial tyrosyl-tRNA synthetase (TyrRS; CYT-18), is bifunctional and both aminoacylates mitochondrial tRNA(Tyr) and promotes the splicing of mitochondrial group I introns. Here we determine a 4.5-A co-crystal structure of the Twort orf142-I2 group I intron ribozyme bound to splicing-active, carboxy-terminally truncated CYT-18. The structure shows that the group I intron binds across the two subunits of the homodimeric protein with a newly evolved RNA-binding surface distinct from that which binds tRNA(Tyr). This RNA binding surface provides an extended scaffold for the phosphodiester backbone of the conserved catalytic core of the intron RNA, allowing the protein to promote the splicing of a wide variety of group I introns. The group I intron-binding surface includes three small insertions and additional structural adaptations relative to non-splicing bacterial TyrRSs, indicating a multistep adaptation for splicing function. The co-crystal structure provides insight into how CYT-18 promotes group I intron splicing, how it evolved to have this function, and how proteins could have incrementally replaced RNA structures during the transition from an RNA world to an RNP world.
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(as it appears on PubMed at http://www.pubmed.gov), where 18172503 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18172503}}
==About this Structure==
==About this Structure==
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[[Category: Rna-protein complex]]
[[Category: Rna-protein complex]]
[[Category: Transit peptide]]
[[Category: Transit peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:05:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:30:22 2008''

Revision as of 18:30, 28 July 2008

Image:2rkj.png

Template:STRUCTURE 2rkj

Cocrystal structure of a tyrosyl-tRNA synthetase splicing factor with a group I intron RNA

Template:ABSTRACT PUBMED 18172503

About this Structure

2RKJ is a Protein complex structure of sequences from Neurospora crassa and Staphylococcus phage twort. Full crystallographic information is available from OCA.

Reference

Structure of a tyrosyl-tRNA synthetase splicing factor bound to a group I intron RNA., Paukstelis PJ, Chen JH, Chase E, Lambowitz AM, Golden BL, Nature. 2008 Jan 3;451(7174):94-7. PMID:18172503

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