2dhc

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{{STRUCTURE_2dhc| PDB=2dhc | SCENE= }}
{{STRUCTURE_2dhc| PDB=2dhc | SCENE= }}
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'''CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE'''
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===CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE===
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==Overview==
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Crystal structures of haloalkane dehalogenase were determined in the presence of the substrate 1,2-dichloroethane. At pH 5 and 4 degrees C, substrate is bound in the active site without being converted; warming to room temperature causes the substrate's carbon-chlorine bond to be broken, producing a chloride ion with concomitant alkylation of the active-site residue Asp124. At pH 6 and room temperature the alkylated enzyme is hydrolysed by a water molecule activated by the His289-Asp260 pair in the active site. These results show that catalysis by the dehalogenase proceeds by a two-step mechanism involving an ester intermediate covalently bound at Asp124.
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(as it appears on PubMed at http://www.pubmed.gov), where 8515812 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8515812}}
==About this Structure==
==About this Structure==
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[[Category: Verschueren, K H.G.]]
[[Category: Verschueren, K H.G.]]
[[Category: Dehalogenase]]
[[Category: Dehalogenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:25:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:32:33 2008''

Revision as of 18:32, 28 July 2008

Image:2dhc.png

Template:STRUCTURE 2dhc

CRYSTALLOGRAPHIC ANALYSIS OF THE CATALYTIC MECHANISM OF HALOALKANE DEHALOGENASE

Template:ABSTRACT PUBMED 8515812

About this Structure

2DHC is a Single protein structure of sequence from Xanthobacter autotrophicus. Full crystallographic information is available from OCA.

Reference

Crystallographic analysis of the catalytic mechanism of haloalkane dehalogenase., Verschueren KH, Seljee F, Rozeboom HJ, Kalk KH, Dijkstra BW, Nature. 1993 Jun 24;363(6431):693-8. PMID:8515812

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