2qsc

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{{STRUCTURE_2qsc| PDB=2qsc | SCENE= }}
{{STRUCTURE_2qsc| PDB=2qsc | SCENE= }}
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'''Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide'''
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===Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide===
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==Overview==
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F425-B4e8 (B4e8) is a monoclonal antibody isolated from a human immunodeficiency virus type 1 (HIV-1)-infected individual that recognizes the V3 variable loop on the gp120 subunit of the viral envelope spike. B4e8 neutralizes a subset of HIV-1 primary isolates from subtypes B, C and D, which places this antibody among the very few human anti-V3 antibodies with notable cross-neutralizing activity. Here, the crystal structure of the B4e8 Fab' fragment in complex with a 24-mer V3 peptide (RP142) at 2.8 A resolution is described. The complex structure reveals that the antibody recognizes a novel V3 loop conformation, featuring a five-residue alpha-turn around the conserved GPGRA apex of the beta-hairpin loop. In agreement with previous mutagenesis analyses, the Fab' interacts primarily with V3 through side-chain contacts with just two residues, Ile(P309) and Arg(P315), while the remaining contacts are to the main chain. The structure helps explain how B4e8 can tolerate a certain degree of sequence variation within V3 and, hence, is able to neutralize an appreciable number of different HIV-1 isolates.
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(as it appears on PubMed at http://www.pubmed.gov), where 18068724 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18068724}}
==About this Structure==
==About this Structure==
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[[Category: Viral immunoevasion]]
[[Category: Viral immunoevasion]]
[[Category: Virion]]
[[Category: Virion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 15:34:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:43:50 2008''

Revision as of 18:43, 28 July 2008

Image:2qsc.png

Template:STRUCTURE 2qsc

Crystal structure analysis of anti-HIV-1 V3-Fab F425-B4e8 in complex with a V3-peptide

Template:ABSTRACT PUBMED 18068724

About this Structure

2QSC is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of antibody F425-B4e8 in complex with a V3 peptide reveals a new binding mode for HIV-1 neutralization., Bell CH, Pantophlet R, Schiefner A, Cavacini LA, Stanfield RL, Burton DR, Wilson IA, J Mol Biol. 2008 Jan 25;375(4):969-78. Epub 2007 Nov 13. PMID:18068724

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