1jxd

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[[Image:1jxd.gif|left|200px]]
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{{Seed}}
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[[Image:1jxd.png|left|200px]]
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{{STRUCTURE_1jxd| PDB=1jxd | SCENE= }}
{{STRUCTURE_1jxd| PDB=1jxd | SCENE= }}
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'''SOLUTION STRUCTURE OF REDUCED CU(I) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803'''
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===SOLUTION STRUCTURE OF REDUCED CU(I) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803===
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==Overview==
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A model-free analysis based on (15)N R(1), (15)N R(2), and (15)N-(1)H nuclear Overhauser effects was performed on reduced (diamagnetic) and oxidized (paramagnetic) forms of plastocyanin from Synechocystis sp. PCC6803. The protein backbone is rigid, displaying a small degree of mobility in the sub-nanosecond time scale. The loops surrounding the copper ion, involved in physiological electron transfer, feature a higher extent of flexibility in the longer time scale in both redox states, as measured from D(2)O exchange of amide protons and from NH-H(2)O saturation transfer experiments. In contrast to the situation for other electron transfer proteins, no significant difference in the dynamic properties is found between the two redox forms. A solution structure was also determined for the reduced plastocyanin and compared with the solution structure of the oxidized form in order to assess possible structural changes related to the copper ion redox state. Within the attained resolution, the structure of the reduced plastocyanin is indistinguishable from that of the oxidized form, even though small chemical shift differences are observed. The present characterization provides information on both the structural and dynamic behavior of blue copper proteins in solution that is useful to understand further the role(s) of protein dynamics in electron transfer processes.
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{{ABSTRACT_PUBMED_11509552}}
==About this Structure==
==About this Structure==
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1JXD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXD OCA].
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1JXD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechocystis_sp. Synechocystis sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXD OCA].
==Reference==
==Reference==
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[[Category: Zhao, J.]]
[[Category: Zhao, J.]]
[[Category: Copper protein beta barrel electron transfer]]
[[Category: Copper protein beta barrel electron transfer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:02:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:46:48 2008''

Revision as of 18:46, 28 July 2008

Image:1jxd.png

Template:STRUCTURE 1jxd

SOLUTION STRUCTURE OF REDUCED CU(I) PLASTOCYANIN FROM SYNECHOCYSTIS PCC6803

Template:ABSTRACT PUBMED 11509552

About this Structure

1JXD is a Single protein structure of sequence from Synechocystis sp.. Full experimental information is available from OCA.

Reference

Backbone dynamics of plastocyanin in both oxidation states. Solution structure of the reduced form and comparison with the oxidized state., Bertini I, Bryant DA, Ciurli S, Dikiy A, Fernandez CO, Luchinat C, Safarov N, Vila AJ, Zhao J, J Biol Chem. 2001 Dec 14;276(50):47217-26. Epub 2001 Aug 16. PMID:11509552

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