1w72

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[[Image:1w72.gif|left|200px]]
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{{STRUCTURE_1w72| PDB=1w72 | SCENE= }}
{{STRUCTURE_1w72| PDB=1w72 | SCENE= }}
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'''CRYSTAL STRUCTURE OF HLA-A1:MAGE-A1 IN COMPLEX WITH FAB-HYB3'''
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===CRYSTAL STRUCTURE OF HLA-A1:MAGE-A1 IN COMPLEX WITH FAB-HYB3===
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==Overview==
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Antibodies with T cell receptor-like specificity possess a considerable diagnostic and therapeutic potential, but the structural basis of the interaction between an antibody and an histocompatibility antigen has so far not been determined. We present here the crystal structure (at 2.15 A resolution) of the recombinant, affinity-matured human antibody fragment Fab-Hyb3 bound to the tumor-associated human leukocyte antigen (HLA)/peptide complex HLA-A1.MAGE-A1. Fab-Hyb3 employs a diagonal docking mode resembling that of T cell receptors. However, other than these natural ligands, the antibody uses only four of its six complementarity-determining regions for direct interactions with the target. It recognizes the C-terminal half of the MAGE-A1 peptide, the HLA-A1 alpha1-helix, and N-terminal residues of the alpha2-helix, accompanied by a large tilting angle between the two types of molecules within the complex. Interestingly, only a single hydrogen bond between a peptide side chain and Fab-Hyb3 contributes to the interaction, but large buried surface areas with pronounced shape complementarity assure high affinity and specificity for MAGE-A1. The HLA-A1.MAGE-A1.antibody structure is discussed in comparison with those of natural ligands recognizing HLA.peptide complexes.
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(as it appears on PubMed at http://www.pubmed.gov), where 15537658 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15537658}}
==About this Structure==
==About this Structure==
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[[Category: Peptide-specific fab]]
[[Category: Peptide-specific fab]]
[[Category: Tcr-like binding]]
[[Category: Tcr-like binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:14:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:10:52 2008''

Revision as of 19:10, 28 July 2008

Image:1w72.png

Template:STRUCTURE 1w72

CRYSTAL STRUCTURE OF HLA-A1:MAGE-A1 IN COMPLEX WITH FAB-HYB3

Template:ABSTRACT PUBMED 15537658

About this Structure

1W72 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

A major histocompatibility complex-peptide-restricted antibody and t cell receptor molecules recognize their target by distinct binding modes: crystal structure of human leukocyte antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3., Hulsmeyer M, Chames P, Hillig RC, Stanfield RL, Held G, Coulie PG, Alings C, Wille G, Saenger W, Uchanska-Ziegler B, Hoogenboom HR, Ziegler A, J Biol Chem. 2005 Jan 28;280(4):2972-80. Epub 2004 Nov 10. PMID:15537658

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