1zue

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[[Image:1zue.gif|left|200px]]
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{{STRUCTURE_1zue| PDB=1zue | SCENE= }}
{{STRUCTURE_1zue| PDB=1zue | SCENE= }}
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'''Revised Solution Structure of DLP-2'''
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===Revised Solution Structure of DLP-2===
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==Overview==
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The recent discovery that the natriuretic peptide OvCNPb (Ornithorhynchus venom C-type natriuretic peptide B) from platypus (Ornithorynchus anatinus) venom contains a D-amino acid residue suggested that other D-amino-acid-containing peptides might be present in the venom. In the present study, we show that DLP-2 (defensin-like peptide-2), a 42-amino-acid residue polypeptide in the platypus venom, also contains a D-amino acid residue, D-methionine, at position 2, while DLP-4, which has an identical amino acid sequence, has all amino acids in the L-form. These findings were supported further by the detection of isomerase activity in the platypus gland venom extract that converts DLP-4 into DLP-2. In the light of this new information, the tertiary structure of DLP-2 was recalculated using a new structural template with D-Met2. The structure of DLP-4 was also determined in order to evaluate the effect of a D-amino acid at position 2 on the structure and possibly to explain the large retention time difference observed for the two molecules in reverse-phase HPLC. The solution structures of the DLP-2 and DLP-4 are very similar to each other and to the earlier reported structure of DLP-2, which assumed that all amino acids were in the L-form. Our results suggest that the incorporation of the D-amino acid at position 2 has minimal effect on the overall fold in solution.
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The line below this paragraph, {{ABSTRACT_PUBMED_16033333}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16033333 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16033333}}
==About this Structure==
==About this Structure==
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1ZUE is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUE OCA].
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1ZUE is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZUE OCA].
==Reference==
==Reference==
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16033333 16033333]
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16033333 16033333]
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Defensin-like peptide-2 from platypus venom: member of a class of peptides with a distinct structural fold., Torres AM, de Plater GM, Doverskog M, Birinyi-Strachan LC, Nicholson GM, Gallagher CH, Kuchel PW, Biochem J. 2000 Jun 15;348 Pt 3:649-56. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10839998 10839998]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Alewood, P F.]]
[[Category: Alewood, P F.]]
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[[Category: Antiparallel beta-sheet]]
[[Category: Antiparallel beta-sheet]]
[[Category: Helix]]
[[Category: Helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:05:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:15:32 2008''

Revision as of 19:15, 28 July 2008

Image:1zue.png

Template:STRUCTURE 1zue

Revised Solution Structure of DLP-2

Template:ABSTRACT PUBMED 16033333

About this Structure

1ZUE is a Single protein structure. Full experimental information is available from OCA.

Reference

D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:16033333

Defensin-like peptide-2 from platypus venom: member of a class of peptides with a distinct structural fold., Torres AM, de Plater GM, Doverskog M, Birinyi-Strachan LC, Nicholson GM, Gallagher CH, Kuchel PW, Biochem J. 2000 Jun 15;348 Pt 3:649-56. PMID:10839998

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