1qxb

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[[Image:1qxb.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1qxb| PDB=1qxb | SCENE= }}
{{STRUCTURE_1qxb| PDB=1qxb | SCENE= }}
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'''NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9'''
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===NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9===
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==Overview==
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A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
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The line below this paragraph, {{ABSTRACT_PUBMED_14627809}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 14627809 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14627809}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXB OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXB OCA].
==Reference==
==Reference==
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[[Category: Disaccharide nucleotide]]
[[Category: Disaccharide nucleotide]]
[[Category: Ribose]]
[[Category: Ribose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:48:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 22:25:46 2008''

Revision as of 19:25, 28 July 2008

Image:1qxb.png

Template:STRUCTURE 1qxb

NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9

Template:ABSTRACT PUBMED 14627809

About this Structure

Full experimental information is available from OCA.

Reference

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:14627809

Page seeded by OCA on Mon Jul 28 22:25:46 2008

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