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1pwk

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{{STRUCTURE_1pwk| PDB=1pwk | SCENE= }}
{{STRUCTURE_1pwk| PDB=1pwk | SCENE= }}
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'''Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of Domain Swapped Dimer Assembly'''
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===Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of Domain Swapped Dimer Assembly===
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==Overview==
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The 8-kDa light chain of dynein (DLC8) is ubiquitously expressed in various cell types. Other than serving as a light chain of the dynein complexes, this highly conserved protein has been shown to bind a larger number of proteins with diverse biological functions. DLC8 forms a homodimer via three-dimensional domain swapping of an internal beta-strand (the beta2-strand) at neutral pH. The protein undergoes non-reversible dimer-to-monomer dissociation when the pH value of the protein solution decreases. The three-dimensional structure of the DLC8 monomer determined by NMR spectroscopy at pH 3.0 showed that the protein is well folded. The major conformational change accompanied by dimer dissociation is in the beta2-strand of the protein, which undergoes transition from a beta-strand to a nascent alpha-helix. The monomer form of DLC8 is not capable of binding to target proteins. Insertion of two flexible amino acid residues in the tight beta1/beta2-loop dramatically stabilized the monomer conformation of the protein. NMR studies showed that the mutation altered the conformation as well as the three-dimensional domain swapping-mediated assembly of the DLC8 dimer. The mutant DLC8 was unable to bind to its targets even at physiological pH. The three-dimensional structure of the mutant protein in its monomeric form provides the structural basis of the mutation-induced stabilization of the monomer conformation. Based on the experimental data, we conclude that the formation of the beta2-strand swapping-mediated dimer is mandatory for the structure and function of DLC8. We further note that the DLC8 dimer represents a novel mode of three-dimensional domain swapping.
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{{ABSTRACT_PUBMED_12904292}}
==About this Structure==
==About this Structure==
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1PWK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PWK OCA].
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1PWK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PWK OCA].
==Reference==
==Reference==
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[[Category: Dynein]]
[[Category: Dynein]]
[[Category: Dynein light chain]]
[[Category: Dynein light chain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:34:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:01:53 2008''

Revision as of 20:02, 28 July 2008

Image:1pwk.png

Template:STRUCTURE 1pwk

Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of Domain Swapped Dimer Assembly

Template:ABSTRACT PUBMED 12904292

About this Structure

1PWK is a Single protein structure of sequence from Rattus norvegicus. Full experimental information is available from OCA.

Reference

Structure of the monomeric 8-kDa dynein light chain and mechanism of the domain-swapped dimer assembly., Wang W, Lo KW, Kan HM, Fan JS, Zhang M, J Biol Chem. 2003 Oct 17;278(42):41491-9. Epub 2003 Aug 6. PMID:12904292

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