2agj

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{{STRUCTURE_2agj| PDB=2agj | SCENE= }}
{{STRUCTURE_2agj| PDB=2agj | SCENE= }}
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'''Crystal Structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody'''
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===Crystal Structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody===
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==Overview==
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The 2.6 A (1 A=0.1 nm) resolution structure has been determined for the glycosylated Fab (fragment antigen binding) of an IgM (Yvo) obtained from a subject with Waldenstrom's macroglobulinaemia. Dynamic light scattering was used to estimate the gel point and monitor the formation of an ordered hydroscopic gel of Yvo IgM upon cooling. If a cryoglobulin forms gels in peripheral tissues and organs, the associated swelling and damage to microvasculature can result in considerable morbidity and mortality. The three-dimensional structure of the branched N-linked oligosaccharide associated with the CH1 domain (first constant domain of heavy chain) is reported. The carbohydrate may act to shield part of the lateral surface of the CH1 domain and crowd the junction between the CH1 and CH2 domains, thereby limiting the segmental flexibility of the Fab arms in intact Yvo IgM, especially at low temperatures. Recently, Yvo IgM was shown to have the properties of a naturally occurring proteolytic antibody [Paul, Karle, Planque, Taguchi, Salas, Nishiyama, Handy, Hunter, Edmundson and Hanson (2004) J. Biol. Chem. 279, 39611-39619; Planque, Bangale, Song, Karle, Taguchi, Poindexter, Bick, Edmundson, Nishiyama and Paul (2004) J. Biol Chem. 279, 14024-14032]. The Yvo protein displayed the ability to cleave, by a nucleophilic mechanism, the amide bonds of a variety of serine protease substrates and the gp120 coat protein of HIV. An atypical serine, arginine and glutamate motif is located in the middle of the Yvo antigen-binding site and displays an overall geometry that mimics the classical serine, histidine and aspartate catalytic triad of serine proteases. Our present findings indicate that pre-existing or natural antibodies can utilize at least one novel strategy for the cleavage of peptide bonds.
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The line below this paragraph, {{ABSTRACT_PUBMED_16422668}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16422668 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16422668}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody., Ramsland PA, Terzyan SS, Cloud G, Bourne CR, Farrugia W, Tribbick G, Geysen HM, Moomaw CR, Slaughter CA, Edmundson AB, Biochem J. 2006 May 1;395(3):473-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16422668 16422668]
Crystal structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody., Ramsland PA, Terzyan SS, Cloud G, Bourne CR, Farrugia W, Tribbick G, Geysen HM, Moomaw CR, Slaughter CA, Edmundson AB, Biochem J. 2006 May 1;395(3):473-81. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16422668 16422668]
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Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains., Shaw DC, Shultz BB, Ramsland PA, Edmundson AB, J Mol Recognit. 2002 Sep-Oct;15(5):341-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12447912 12447912]
[[Category: Bourne, C R.]]
[[Category: Bourne, C R.]]
[[Category: Cloud, G.]]
[[Category: Cloud, G.]]
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[[Category: Catalytic antibody]]
[[Category: Catalytic antibody]]
[[Category: Immunoglobulin superfamily]]
[[Category: Immunoglobulin superfamily]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:01:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:03:05 2008''

Revision as of 20:03, 28 July 2008

Image:2agj.png

Template:STRUCTURE 2agj

Crystal Structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody

Template:ABSTRACT PUBMED 16422668

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of a glycosylated Fab from an IgM cryoglobulin with properties of a natural proteolytic antibody., Ramsland PA, Terzyan SS, Cloud G, Bourne CR, Farrugia W, Tribbick G, Geysen HM, Moomaw CR, Slaughter CA, Edmundson AB, Biochem J. 2006 May 1;395(3):473-81. PMID:16422668

Dealing with intractable protein cores: protein sequencing of the Mcg IgG and the Yvo IgM heavy chain variable domains., Shaw DC, Shultz BB, Ramsland PA, Edmundson AB, J Mol Recognit. 2002 Sep-Oct;15(5):341-8. PMID:12447912

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