1ofk

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{{STRUCTURE_1ofk| PDB=1ofk | SCENE= }}
{{STRUCTURE_1ofk| PDB=1ofk | SCENE= }}
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'''RECOMBINANT SPERM WHALE MYOGLOBIN F43H, H64L MUTANT (MET)'''
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===RECOMBINANT SPERM WHALE MYOGLOBIN F43H, H64L MUTANT (MET)===
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==Overview==
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To clarify how the location of distal histidine affects the activation process of H2O2 by heme proteins, we have characterized reactions with H2O2 for the L29H/H64L and F43H/H64L mutants of sperm whale myoglobin (Mb), designed to locate the histidine farther from the heme iron. Whereas the L29H/H64L double substitution retarded the reaction with H2O2, an 11-fold rate increase versus wild-type Mb was observed for the F43H/H64L mutant. The Vmax values for 1-electron oxidations by the myoglobins correlate well with the varied reactivities with H2O2. The functions of the distal histidine as a general acid-base catalyst were examined based on the reactions with cumene hydroperoxide and cyanide, and only the histidine in F43H/H64L Mb was suggested to facilitate heterolysis of the peroxide bond. The x-ray crystal structures of the mutants confirmed that the distal histidines in F43H/H64L Mb and peroxidase are similar in distance from the heme iron, whereas the distal histidine in L29H/H64L Mb is located too far to enhance heterolysis. Our results indicate that the proper positioning of the distal histidine is essential for the activation of H2O2 by heme enzymes.
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(as it appears on PubMed at http://www.pubmed.gov), where 9915818 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9915818}}
==About this Structure==
==About this Structure==
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[[Category: Oxygen transport]]
[[Category: Oxygen transport]]
[[Category: Peroxidase activity]]
[[Category: Peroxidase activity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:47:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:06:52 2008''

Revision as of 20:06, 28 July 2008

Image:1ofk.png

Template:STRUCTURE 1ofk

RECOMBINANT SPERM WHALE MYOGLOBIN F43H, H64L MUTANT (MET)

Template:ABSTRACT PUBMED 9915818

About this Structure

1OFK is a Single protein structure of sequence from Physeter catodon. Full crystallographic information is available from OCA.

Reference

Effects of the location of distal histidine in the reaction of myoglobin with hydrogen peroxide., Matsui T, Ozaki S, Liong E, Phillips GN Jr, Watanabe Y, J Biol Chem. 1999 Jan 29;274(5):2838-44. PMID:9915818

Page seeded by OCA on Mon Jul 28 23:06:52 2008

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