2bfn

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{{STRUCTURE_2bfn| PDB=2bfn | SCENE= }}
{{STRUCTURE_2bfn| PDB=2bfn | SCENE= }}
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'''THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.'''
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===THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.===
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==Overview==
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1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been considered inactive with TCP, since the reaction cannot be easily detected by conventional analytical methods. Here we demonstrate detection of the weak activity (k(cat) = 0.005 s(-1)) of LinB with TCP using X-ray crystallography and microcalorimetry. This observation makes LinB a useful starting material for the development of a new biocatalyst toward TCP by protein engineering. Microcalorimetry is proposed to be a universal method for the detection of weak enzymatic activities. Detection of these activities is becoming increasingly important for engineering novel biocatalysts using the scaffolds of proteins with promiscuous activities.
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(as it appears on PubMed at http://www.pubmed.gov), where 17259360 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17259360}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17259360 17259360]
Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17259360 17259360]
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Crystal structure of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26., Marek J, Vevodova J, Smatanova IK, Nagata Y, Svensson LA, Newman J, Takagi M, Damborsky J, Biochemistry. 2000 Nov 21;39(46):14082-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11087355 11087355]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Banas, P.]]
[[Category: Banas, P.]]
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[[Category: Haloalkane dehalogenase linb]]
[[Category: Haloalkane dehalogenase linb]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:13:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:10:04 2008''

Revision as of 20:10, 28 July 2008

Image:2bfn.png

Template:STRUCTURE 2bfn

THE CRYSTAL STRUCTURE OF THE COMPLEX OF THE HALOALKANE DEHALOGENASE LINB WITH THE PRODUCT OF DEHALOGENATION REACTION 1,2-DICHLOROPROPANE.

Template:ABSTRACT PUBMED 17259360

About this Structure

2BFN is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Weak activity of haloalkane dehalogenase LinB with 1,2,3-trichloropropane revealed by X-Ray crystallography and microcalorimetry., Monincova M, Prokop Z, Vevodova J, Nagata Y, Damborsky J, Appl Environ Microbiol. 2007 Mar;73(6):2005-8. Epub 2007 Jan 26. PMID:17259360

Crystal structure of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26., Marek J, Vevodova J, Smatanova IK, Nagata Y, Svensson LA, Newman J, Takagi M, Damborsky J, Biochemistry. 2000 Nov 21;39(46):14082-6. PMID:11087355

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