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2cyu

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[[Image:2cyu.gif|left|200px]]
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{{STRUCTURE_2cyu| PDB=2cyu | SCENE= }}
{{STRUCTURE_2cyu| PDB=2cyu | SCENE= }}
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'''NMR structure of a downhill folding protein'''
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===NMR structure of a downhill folding protein===
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==Overview==
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Protein folding is an inherently complex process involving coordination of the intricate networks of weak interactions that stabilize native three-dimensional structures. In the conventional paradigm, simple protein structures are assumed to fold in an all-or-none process that is inaccessible to experiment. Existing experimental methods therefore probe folding mechanisms indirectly. A widely used approach interprets changes in protein stability and/or folding kinetics, induced by engineered mutations, in terms of the structure of the native protein. In addition to limitations in connecting energetics with structure, mutational methods have significant experimental uncertainties and are unable to map complex networks of interactions. In contrast, analytical theory predicts small barriers to folding and the possibility of downhill folding. These theoretical predictions have been confirmed experimentally in recent years, including the observation of global downhill folding. However, a key remaining question is whether downhill folding can indeed lead to the high-resolution analysis of protein folding processes. Here we show, with the use of nuclear magnetic resonance (NMR), that the downhill protein BBL from Escherichia coli unfolds atom by atom starting from a defined three-dimensional structure. Thermal unfolding data on 158 backbone and side-chain protons out of a total of 204 provide a detailed view of the structural events during folding. This view confirms the statistical nature of folding, and exposes the interplay between hydrogen bonding, hydrophobic forces, backbone conformation and side-chain entropy. From the data we also obtain a map of the interaction network in this protein, which reveals the source of folding cooperativity. Our approach can be extended to other proteins with marginal barriers (less than 3RT), providing a new tool for the study of protein folding.
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(as it appears on PubMed at http://www.pubmed.gov), where 16799571 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16799571}}
==About this Structure==
==About this Structure==
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2CYU is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CYU OCA].
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2CYU is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CYU OCA].
==Reference==
==Reference==
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[[Category: Downhill folding protein]]
[[Category: Downhill folding protein]]
[[Category: Helix bundle]]
[[Category: Helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:22:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:11:28 2008''

Revision as of 20:11, 28 July 2008

Image:2cyu.png

Template:STRUCTURE 2cyu

NMR structure of a downhill folding protein

Template:ABSTRACT PUBMED 16799571

About this Structure

2CYU is a Single protein structure. Full experimental information is available from OCA.

Reference

Atom-by-atom analysis of global downhill protein folding., Sadqi M, Fushman D, Munoz V, Nature. 2006 Jul 20;442(7100):317-21. Epub 2006 Jun 14. PMID:16799571

Page seeded by OCA on Mon Jul 28 23:11:28 2008

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