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1p4b

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[[Image:1p4b.gif|left|200px]]
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{{STRUCTURE_1p4b| PDB=1p4b | SCENE= }}
{{STRUCTURE_1p4b| PDB=1p4b | SCENE= }}
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'''Three-Dimensional Structure Of a Single Chain Fv Fragment Complexed With The peptide GCN4(7P-14P).'''
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===Three-Dimensional Structure Of a Single Chain Fv Fragment Complexed With The peptide GCN4(7P-14P).===
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==Overview==
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We generated a single chain Fv fragment of an antibody (scFv) with a binding affinity of about 5 pm to a short peptide by applying rigorous directed evolution. Starting from a high affinity peptide binder, originally obtained by ribosome display from a murine library, we generated libraries of mutants with error-prone PCR and DNA shuffling and applied off-rate selection by using ribosome display. Crystallographic analysis of the scFv in its antigen-bound and free state showed that only few mutations, which do not make direct contact to the antigen, lead to a 500-fold affinity improvement over its potential germ line precursor. These results suggest that the affinity optimization of very high affinity binders is achieved by modulating existing interactions via subtle changes in the framework rather than by introducing new contacts. Off-rate selection in combination with ribosome display can evolve binders to the low picomolar affinity range even for peptide targets.
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(as it appears on PubMed at http://www.pubmed.gov), where 14754898 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14754898}}
==About this Structure==
==About this Structure==
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[[Category: Picomolar binder]]
[[Category: Picomolar binder]]
[[Category: Scfv]]
[[Category: Scfv]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:40:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:13:09 2008''

Revision as of 20:13, 28 July 2008

Image:1p4b.png

Template:STRUCTURE 1p4b

Three-Dimensional Structure Of a Single Chain Fv Fragment Complexed With The peptide GCN4(7P-14P).

Template:ABSTRACT PUBMED 14754898

About this Structure

1P4B is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity., Zahnd C, Spinelli S, Luginbuhl B, Amstutz P, Cambillau C, Pluckthun A, J Biol Chem. 2004 Apr 30;279(18):18870-7. Epub 2004 Jan 30. PMID:14754898

Page seeded by OCA on Mon Jul 28 23:13:09 2008

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