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| | {{STRUCTURE_1rro| PDB=1rro | SCENE= }} | | {{STRUCTURE_1rro| PDB=1rro | SCENE= }} |
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| - | '''REFINEMENT OF RECOMBINANT ONCOMODULIN AT 1.30 ANGSTROMS RESOLUTION'''
| + | ===REFINEMENT OF RECOMBINANT ONCOMODULIN AT 1.30 ANGSTROMS RESOLUTION=== |
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| - | ==Overview==
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| - | A refinement of the oncomodulin crystal structure at 1.30 A resolution has been carried out with X-ray data from the recombinant protein. The crystallographic R-factor values are 0.169 for 19,995 reflections in the range 6.0 to 1.30 A, which were used for the restrained least-squares refinement, and 0.176 for 20,186 observed reflections in the range 10.0 to 1.30 A. This high resolution refinement has enabled us to make more definitive statements about the molecular structure than was possible heretofore. The present model includes residues 1 to 108, the two Ca2+ of the CD and EF loops, two intermolecular Ca2+, and 103 water molecules per oncomodulin molecule. The electron density maps indicate disordered orientations for ten residues on the hydrophilic surface of the molecule. The pattern of molecular aggregation via intermolecular Ca2+, which occurs in the native rat oncomodulin structure, is also present in the recombinant oncomodulin structure. The Cys18 side-chain is not in a position that would be easily accessible for molecular dimerization via a disulphide bond. The substitution of Glu59, which is preserved in all the determined species of parvalbumin, by Asp59 in oncomodulin seems to break a stabilizing hydrogen bond in the CD loop and render the main-chain in positions 59 to 60 somewhat unstable. This instability in the CD loop, and the strong tendency of oncomodulin for molecular aggregation via intermolecular Ca2+, appear to be the two outstanding features that may account for oncomodulin's biological peculiarities.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8487302}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8487302 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8487302}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: To, R.]] | | [[Category: To, R.]] |
| | [[Category: Calcium-binding protein]] | | [[Category: Calcium-binding protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:50:01 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:13:58 2008'' |
Revision as of 20:14, 28 July 2008
Template:STRUCTURE 1rro
REFINEMENT OF RECOMBINANT ONCOMODULIN AT 1.30 ANGSTROMS RESOLUTION
Template:ABSTRACT PUBMED 8487302
About this Structure
1RRO is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Refinement of recombinant oncomodulin at 1.30 A resolution., Ahmed FR, Rose DR, Evans SV, Pippy ME, To R, J Mol Biol. 1993 Apr 20;230(4):1216-24. PMID:8487302
Page seeded by OCA on Mon Jul 28 23:13:58 2008
