From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2o8v.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2o8v.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2o8v| PDB=2o8v | SCENE= }} | | {{STRUCTURE_2o8v| PDB=2o8v | SCENE= }} |
| | | | |
| - | '''PAPS reductase in a covalent complex with thioredoxin C35A'''
| + | ===PAPS reductase in a covalent complex with thioredoxin C35A=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The crystal structure of Escherichia coli 3'-phosphoadenosine-5'-phosphosulfate (PAPS) reductase in complex with E. coli thioredoxin 1 (Trx1) has been determined to 3.0 A resolution. The two proteins are covalently linked via a mixed disulfide that forms during nucleophilic attack of Trx's N-terminal cysteine on the Sgamma atom of the PAPS reductase S-sulfocysteine (E-Cys-Sgamma-SO3-), a central intermediate in the catalytic cycle. For the first time in a crystal structure, residues 235-244 in the PAPS reductase C-terminus are observed, depicting an array of interprotein salt bridges between Trx and the strictly conserved glutathione-like sequence, Glu238Cys239Gly240Leu241His242. The structure also reveals a Trx-binding surface adjacent to the active site cleft and regions of PAPS reductase associated with conformational change. Interaction at this site strategically positions Trx to bind the S-sulfated C-terminus and addresses the mechanism for requisite structural rearrangement of this domain. An apparent sulfite-binding pocket at the protein-protein interface explicitly orients the S-sulfocysteine Sgamma atom for nucleophilic attack in a subsequent step. Taken together, the structure of PAPS reductase in complex with Trx highlights the large structural rearrangement required to accomplish sulfonucleotide reduction and suggests a role for Trx in catalysis beyond the paradigm of disulfide reduction.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17352498}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17352498 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_17352498}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 27: |
Line 31: |
| | [[Category: Stout, C D.]] | | [[Category: Stout, C D.]] |
| | [[Category: Disulfide crosslinked complex]] | | [[Category: Disulfide crosslinked complex]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 10:28:14 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:15:06 2008'' |
Revision as of 20:15, 28 July 2008
Template:STRUCTURE 2o8v
PAPS reductase in a covalent complex with thioredoxin C35A
Template:ABSTRACT PUBMED 17352498
About this Structure
2O8V is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
3'-Phosphoadenosine-5'-phosphosulfate reductase in complex with thioredoxin: a structural snapshot in the catalytic cycle., Chartron J, Shiau C, Stout CD, Carroll KS, Biochemistry. 2007 Apr 3;46(13):3942-51. Epub 2007 Mar 13. PMID:17352498
Page seeded by OCA on Mon Jul 28 23:15:06 2008
