2v3b

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{{STRUCTURE_2v3b| PDB=2v3b | SCENE= }}
{{STRUCTURE_2v3b| PDB=2v3b | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE ELECTRON TRANSFER COMPLEX RUBREDOXIN- RUBREDOXIN REDUCTASE FROM PSEUDOMONAS AERUGINOSA.'''
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===CRYSTAL STRUCTURE OF THE ELECTRON TRANSFER COMPLEX RUBREDOXIN- RUBREDOXIN REDUCTASE FROM PSEUDOMONAS AERUGINOSA.===
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==Overview==
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Crude oil spills represent a major ecological threat because of the chemical inertness of the constituent n-alkanes. The Gram-negative bacterium Pseudomonas aeruginosa is one of the few bacterial species able to metabolize such compounds. Three chromosomal genes, rubB, rubA1, and rubA2 coding for an NAD(P)H:rubredoxin reductase (RdxR) and two rubredoxins (Rdxs) are indispensable for this ability. They constitute an electron transport (ET) pathway that shuttles reducing equivalents from carbon metabolism to the membrane-bound alkane hydroxylases AlkB1 and AlkB2. The RdxR-Rdx system also is crucial as part of the oxidative stress response in archaea or anaerobic bacteria. The redox couple has been analyzed in detail as a model system for ET processes. We have solved the structure of RdxR of P. aeruginosa both alone and in complex with Rdx, without the need for cross-linking, and both structures were refined at 2.40- and 2.45-A resolution, respectively. RdxR consists of two cofactor-binding domains and a C-terminal domain essential for the specific recognition of Rdx. Only a small number of direct interactions govern mutual recognition of RdxR and Rdx, corroborating the transient nature of the complex. The shortest distance between the redox centers is observed to be 6.2 A.
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(as it appears on PubMed at http://www.pubmed.gov), where 17636129 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17636129}}
==About this Structure==
==About this Structure==
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[[Category: Nad]]
[[Category: Nad]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 18:08:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:21:51 2008''

Revision as of 20:22, 28 July 2008

Image:2v3b.png

Template:STRUCTURE 2v3b

CRYSTAL STRUCTURE OF THE ELECTRON TRANSFER COMPLEX RUBREDOXIN- RUBREDOXIN REDUCTASE FROM PSEUDOMONAS AERUGINOSA.

Template:ABSTRACT PUBMED 17636129

About this Structure

2V3B is a Protein complex structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of the electron transfer complex rubredoxin rubredoxin reductase of Pseudomonas aeruginosa., Hagelueken G, Wiehlmann L, Adams TM, Kolmar H, Heinz DW, Tummler B, Schubert WD, Proc Natl Acad Sci U S A. 2007 Jul 24;104(30):12276-81. Epub 2007 Jul 16. PMID:17636129

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