1o7x
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(New page: 200px<br /><applet load="1o7x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1o7x, resolution 2.7Å" /> '''CITRATE SYNTHASE FROM...)
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Revision as of 20:44, 20 November 2007
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CITRATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS
Overview
The crystal structure of citrate synthase from the thermophilic Archaeon, Sulfolobus solfataricus (optimum growth temperature = 85 degrees C) has, been determined, extending the number of crystal structures of citrate, synthase from different organisms to a total of five that span the, temperature range over which life exists (from psychrophile to, hyperthermophile). Detailed structural analysis has revealed possible, molecular mechanisms that determine the different stabilities of the five, proteins. The key to these mechanisms is the precise structural location, of the additional interactions. As one ascends the temperature ladder, the, subunit interface of this dimeric enzyme and loop regions are reinforced, by complex electrostatic interactions, and there is a reduced exposure of, hydrophobic surface. These observations reveal a progressive pattern of, stabilization through multiple additional interactions at solvent exposed, loop and interfacial regions.
About this Structure
1O7X is a Single protein structure of sequence from Sulfolobus solfataricus. Active as Citrate (Si)-synthase, with EC number 2.3.3.1 Full crystallographic information is available from OCA.
Reference
Stepwise adaptations of citrate synthase to survival at life's extremes. From psychrophile to hyperthermophile., Bell GS, Russell RJ, Connaris H, Hough DW, Danson MJ, Taylor GL, Eur J Biochem. 2002 Dec;269(24):6250-60. PMID:12473121
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