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1y7y

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{{STRUCTURE_1y7y| PDB=1y7y | SCENE= }}
{{STRUCTURE_1y7y| PDB=1y7y | SCENE= }}
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'''High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila'''
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===High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila===
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==Overview==
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Restriction-modification (R-M) systems serve to protect the host bacterium from invading bacteriophage. The multi-component system includes a methyltransferase, which recognizes and methylates a specific DNA sequence, and an endonuclease which recognises the same sequence and cleaves within or close to this site. The endonuclease will only cleave DNA that is unmethylated at the specific site, thus host DNA is protected while non-host DNA is cleaved. However, following DNA replication, expression of the endonuclease must be delayed until the host DNA is appropriately methylated. In many R-M systems, this regulation is achieved at the transcriptional level via the controller protein, or C-protein. We have solved the first X-ray structure of an R-M controller protein, C.AhdI, to 1.69 A resolution using selenomethionine MAD. C.AhdI is part of a Type IIH R-M system from the pathogen Aeromonas hydrophila. The structure reveals an all-alpha protein that contains a classical helix-turn-helix (HTH) domain and can be assigned to the Xre family of transcriptional regulators. Unlike its monomeric structural homologues, an extended helix generates an interface that results in dimerisation of the free protein. The dimer is electrostatically polarised and a positively charged surface corresponds to the position of the DNA recognition helices of the HTH domain. Comparison with the structure of the lambda cI ternary complex suggests that C.AhdI activates transcription through direct contact with the sigma70 subunit of RNA polymerase.
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(as it appears on PubMed at http://www.pubmed.gov), where 15713456 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15713456}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila., McGeehan JE, Streeter SD, Papapanagiotou I, Fox GC, Kneale GG, J Mol Biol. 2005 Feb 25;346(3):689-701. Epub 2005 Jan 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713456 15713456]
High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila., McGeehan JE, Streeter SD, Papapanagiotou I, Fox GC, Kneale GG, J Mol Biol. 2005 Feb 25;346(3):689-701. Epub 2005 Jan 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15713456 15713456]
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Crystallization and preliminary X-ray analysis of the controller protein C.AhdI from Aeromonas hydrophilia., McGeehan JE, Streeter S, Cooper JB, Mohammed F, Fox GC, Kneale GG, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):323-5. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747712 14747712]
[[Category: Aeromonas hydrophila]]
[[Category: Aeromonas hydrophila]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Helix-turn-helix]]
[[Category: Helix-turn-helix]]
[[Category: Transcriptional regulator]]
[[Category: Transcriptional regulator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:58:57 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:38:52 2008''

Revision as of 20:38, 28 July 2008

Image:1y7y.png

Template:STRUCTURE 1y7y

High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila

Template:ABSTRACT PUBMED 15713456

About this Structure

1Y7Y is a Single protein structure of sequence from Aeromonas hydrophila. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structure of the restriction-modification controller protein C.AhdI from Aeromonas hydrophila., McGeehan JE, Streeter SD, Papapanagiotou I, Fox GC, Kneale GG, J Mol Biol. 2005 Feb 25;346(3):689-701. Epub 2005 Jan 12. PMID:15713456

Crystallization and preliminary X-ray analysis of the controller protein C.AhdI from Aeromonas hydrophilia., McGeehan JE, Streeter S, Cooper JB, Mohammed F, Fox GC, Kneale GG, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):323-5. Epub 2004, Jan 23. PMID:14747712

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