1siy

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[[Image:1siy.gif|left|200px]]
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{{STRUCTURE_1siy| PDB=1siy | SCENE= }}
{{STRUCTURE_1siy| PDB=1siy | SCENE= }}
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'''NMR structure of mung bean non-specific lipid transfer protein 1'''
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===NMR structure of mung bean non-specific lipid transfer protein 1===
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==Overview==
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Plant nonspecific lipid transfer proteins (nsLTPs) are thermal stable proteins that are capable of transferring lipid molecules between bilayers in vitro. This family of proteins, abundant in plants, is proposed to be involved in defense, pollination, and germination; the in vivo biological function remains, however, elusive. Here we report the purification and sequencing of an nsLTP1 from mung bean sprouts. We have also determined the solution structure of this nsLTP1, which represents the first 3D structure of the dicotyledonous nsLTP1 family. The global fold of mung bean nsLTP1 is similar to those of the monocotyledonous nsLTP1 structures and consists of four alpha-helices stabilized by four disulfide bonds. There are, however, some notable differences in the C-terminal tails and internal hydrophobic cavities. Circular dichroism and fluorescence spectroscopy were used to compare the thermodynamics and lipid transfer properties of mung bean nsLTP1 with those of rice nsLTP1. Docking of a lipid molecule into the solution structure of mung bean nsLTP1 reveals similar binding cavities and hydrophobic interactions as in rice nsLTP1, consistent with their comparable lipid transfer properties measured experimentally.
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(as it appears on PubMed at http://www.pubmed.gov), where 15823028 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15823028}}
==About this Structure==
==About this Structure==
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1SIY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vigna_radiata_var._radiata Vigna radiata var. radiata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIY OCA].
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1SIY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vigna_radiata_var._radiata Vigna radiata var. radiata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIY OCA].
==Reference==
==Reference==
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[[Category: Samuel, D.]]
[[Category: Samuel, D.]]
[[Category: Alpha helix]]
[[Category: Alpha helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:45:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:41:08 2008''

Revision as of 20:41, 28 July 2008

Image:1siy.png

Template:STRUCTURE 1siy

NMR structure of mung bean non-specific lipid transfer protein 1

Template:ABSTRACT PUBMED 15823028

About this Structure

1SIY is a Single protein structure of sequence from Vigna radiata var. radiata. Full experimental information is available from OCA.

Reference

Characterization and structural analyses of nonspecific lipid transfer protein 1 from mung bean., Lin KF, Liu YN, Hsu ST, Samuel D, Cheng CS, Bonvin AM, Lyu PC, Biochemistry. 2005 Apr 19;44(15):5703-12. PMID:15823028

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