1obu
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(New page: 200px<br /><applet load="1obu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1obu, resolution 2.00Å" /> '''APOCRUSTACYANIN C1 C...)
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Revision as of 20:47, 20 November 2007
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APOCRUSTACYANIN C1 CRYSTALS GROWN IN SPACE AND EARTH USING VAPOUR DIFFUSION GEOMETRY
Overview
Models of apocrustacyanin C(1) were refined against X-ray data recorded on, Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a, space-grown and an earth-grown crystal, respectively, both using, vapour-diffusion crystal-growth geometry. The space crystals were grown in, the APCF on the NASA Space Shuttle. The microgravity crystal growth showed, a cyclic nature attributed to Marangoni convection, thus reducing the, benefits of the microgravity environment, as reported previously [Chayen, et al. (1996), Q. Rev. Biophys. 29, 227-278]. A subsequent mosaicity, evaluation, also reported previously, showed only a partial improvement in, the space-grown crystals over the earth-grown crystals [Snell et al., (1997), Acta Cryst. D53, 231-239], contrary to the case for lysozyme, crystals grown in space with liquid-liquid diffusion, i.e. without any, major motion during growth [Snell et al. (1995), Acta Cryst. D52, 1099-1102]. In this paper, apocrustacyanin C(1) electron-density maps from, the two refined models are now compared. It is concluded that the, electron-density maps of the protein and the bound waters are found to be, better overall for the structures of apocrustacyanin C(1) studied from the, space-grown crystal compared with those from the earth-grown crystal, even, though both crystals were grown using vapour-diffusion crystal-growth, geometry. The improved residues are on the surface of the protein, with, two involved in or nearby crystal lattice-forming interactions, thus, linking an improved crystal-growth mechanism to the molecular level. The, structural comparison procedures developed should themselves be valuable, for evaluating crystal-growth procedures in the future.
About this Structure
1OBU is a Single protein structure of sequence from Homarus gammarus. Full crystallographic information is available from OCA.
Reference
Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons., Habash J, Boggon TJ, Raftery J, Chayen NE, Zagalsky PF, Helliwell JR, Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1117-23. Epub 2003, Jun 27. PMID:12832753
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