From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2g13.jpg|left|200px]] | + | {{Seed}} |
| | + | [[Image:2g13.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2g13| PDB=2g13 | SCENE= }} | | {{STRUCTURE_2g13| PDB=2g13 | SCENE= }} |
| | | | |
| - | '''CsoS1A with sulfate ion'''
| + | ===CsoS1A with sulfate ion=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The carboxysome is a bacterial organelle that functions to enhance the efficiency of CO2 fixation by encapsulating the enzymes ribulose bisphosphate carboxylase/oxygenase (RuBisCO) and carbonic anhydrase. The outer shell of the carboxysome is reminiscent of a viral capsid, being constructed from many copies of a few small proteins. Here we describe the structure of the shell protein CsoS1A from the chemoautotrophic bacterium Halothiobacillus neapolitanus. The CsoS1A protein forms hexameric units that pack tightly together to form a molecular layer, which is perforated by narrow pores. Sulfate ions, soaked into crystals of CsoS1A, are observed in the pores of the molecular layer, supporting the idea that the pores could be the conduit for negatively charged metabolites such as bicarbonate, which must cross the shell. The problem of diffusion across a semiporous protein shell is discussed, with the conclusion that the shell is sufficiently porous to allow adequate transport of small molecules. The molecular layer formed by CsoS1A is similar to the recently observed layers formed by cyanobacterial carboxysome shell proteins. This similarity supports the argument that the layers observed represent the natural structure of the facets of the carboxysome shell. Insights into carboxysome function are provided by comparisons of the carboxysome shell to viral capsids, and a comparison of its pores to the pores of transmembrane protein channels. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17518518}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17518518 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_17518518}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 29: |
Line 33: |
| | [[Category: Yeates, T O.]] | | [[Category: Yeates, T O.]] |
| | [[Category: Bacterial microcompartment domain]] | | [[Category: Bacterial microcompartment domain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:34:37 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:49:55 2008'' |
Revision as of 20:49, 28 July 2008
Template:STRUCTURE 2g13
CsoS1A with sulfate ion
Template:ABSTRACT PUBMED 17518518
About this Structure
2G13 is a Single protein structure of sequence from Halothiobacillus neapolitanus. Full crystallographic information is available from OCA.
Reference
Structural analysis of CsoS1A and the protein shell of the Halothiobacillus neapolitanus carboxysome., Tsai Y, Sawaya MR, Cannon GC, Cai F, Williams EB, Heinhorst S, Kerfeld CA, Yeates TO, PLoS Biol. 2007 Jun;5(6):e144. PMID:17518518
Page seeded by OCA on Mon Jul 28 23:49:55 2008
