1t2t

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{{STRUCTURE_1t2t| PDB=1t2t | SCENE= }}
{{STRUCTURE_1t2t| PDB=1t2t | SCENE= }}
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'''Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site'''
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===Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site===
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==Overview==
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Customary binding sites of intron-encoded homing endonucleases lie within cognate intronless alleles, at the so-called homing sites. Here, we describe a novel, high-affinity binding site for I-TevI endonuclease, encoded within the group I td intron of phage T4. This site is an operator that overlaps the T4 late promoter, which drives I-TevI expression from within the td intron. I-TevI binds the operator and homing sites with equal affinity, and functions as a transcriptional autorepressor. Distinct sequence and spacing requirements of the catalytic domain result in reduced cleavage activity on operator DNA. Crystallographic studies showed that the overall interactions of the DNA-binding domain with the operator and homing sites are similar, but have some different hydrogen-bonding contacts. We present a model in which the flexibility in protein-DNA interactions allows I-TevI to bind variant intronless alleles to promote intron mobility while facilitating its function in autorepression, and thereby persistence in its host.
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==About this Structure==
==About this Structure==
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[[Category: Stanger, M J.]]
[[Category: Stanger, M J.]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:50:41 2008''

Revision as of 20:50, 28 July 2008

Image:1t2t.png

Template:STRUCTURE 1t2t

Crystal structure of the DNA-binding domain of intron endonuclease I-TevI with operator site

Template:ABSTRACT PUBMED 15361856

About this Structure

1T2T is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.

Reference

Intron-encoded homing endonuclease I-TevI also functions as a transcriptional autorepressor., Edgell DR, Derbyshire V, Van Roey P, LaBonne S, Stanger MJ, Li Z, Boyd TM, Shub DA, Belfort M, Nat Struct Mol Biol. 2004 Oct;11(10):936-44. Epub 2004 Sep 7. PMID:15361856

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