2ixs

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[[Image:2ixs.gif|left|200px]]
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{{STRUCTURE_2ixs| PDB=2ixs | SCENE= }}
{{STRUCTURE_2ixs| PDB=2ixs | SCENE= }}
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'''STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE'''
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===STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE===
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==Overview==
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Rare-cutting restriction enzymes are important tools in genome analysis. We report here the crystal structure of SdaI restriction endonuclease, which is specific for the 8 bp sequence CCTGCA/GG ("/" designates the cleavage site). Unlike orthodox Type IIP enzymes, which are single domain proteins, the SdaI monomer is composed of two structural domains. The N domain contains a classical winged helix-turn-helix (wHTH) DNA binding motif, while the C domain shows a typical restriction endonuclease fold. The active site of SdaI is located within the C domain and represents a variant of the canonical PD-(D/E)XK motif. SdaI determinants of sequence specificity are clustered on the recognition helix of the wHTH motif at the N domain. The modular architecture of SdaI, wherein one domain mediates DNA binding while the other domain is predicted to catalyze hydrolysis, distinguishes SdaI from previously characterized restriction enzymes interacting with symmetric recognition sequences.
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(as it appears on PubMed at http://www.pubmed.gov), where 16962970 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16962970}}
==About this Structure==
==About this Structure==
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[[Category: Restriction endonuclease]]
[[Category: Restriction endonuclease]]
[[Category: Sdai]]
[[Category: Sdai]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:03:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:53:05 2008''

Revision as of 20:53, 28 July 2008

Image:2ixs.png

Template:STRUCTURE 2ixs

STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE

Template:ABSTRACT PUBMED 16962970

About this Structure

2IXS is a Single protein structure of sequence from Streptomyces diastaticus. Full crystallographic information is available from OCA.

Reference

The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:16962970

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