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2gcq

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{{STRUCTURE_2gcq| PDB=2gcq | SCENE= }}
{{STRUCTURE_2gcq| PDB=2gcq | SCENE= }}
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'''Fully ligated E.Coli Adenylosuccinate Synthetase with GTP, 2'-deoxy-IMP and Hadacidin'''
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===Fully ligated E.Coli Adenylosuccinate Synthetase with GTP, 2'-deoxy-IMP and Hadacidin===
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==Overview==
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Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, coupling L-aspartate and IMP to form adenylosuccinate. Km values of IMP and 2'-deoxy-IMP are nearly identical with each substrate supporting comparable maximal velocities. Nonetheless, the Km value for L-aspartate and the Ki value for hadacidin (a competitive inhibitor with respect to L-aspartate) are 29-57-fold lower in the presence of IMP than in the presence of 2'-deoxy-IMP. Crystal structures of the synthetase ligated with hadacidin, GDP, and either 6-phosphoryl-IMP or 2'-deoxy-6-phosphoryl-IMP are identical except for the presence of a cavity normally occupied by the 2'-hydroxyl group of IMP. In the presence of 6-phosphoryl-IMP and GDP (hadacidin absent), the L-aspartate pocket can retain its fully ligated conformation, forming hydrogen bonds between the 2'-hydroxyl group of IMP and sequence-invariant residues. In the presence of 2'-deoxy-6-phosphoryl-IMP and GDP, however, the L-aspartate pocket is poorly ordered. The absence of the 2'-hydroxyl group of the deoxyribonucleotide may destabilize binding of the ligand to the L-aspartate pocket by disrupting hydrogen bonds that maintain a favorable protein conformation and by the introduction of a cavity into the fully ligated active site. At an approximate energy cost of 2.2 kcal/mol, the unfavorable thermodynamics of cavity formation may be the major factor in destabilizing ligands at the L-aspartate pocket.
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(as it appears on PubMed at http://www.pubmed.gov), where 16981730 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16981730}}
==About this Structure==
==About this Structure==
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[[Category: Gtp]]
[[Category: Gtp]]
[[Category: Hadacidin]]
[[Category: Hadacidin]]
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Revision as of 21:03, 28 July 2008

Image:2gcq.png

Template:STRUCTURE 2gcq

Fully ligated E.Coli Adenylosuccinate Synthetase with GTP, 2'-deoxy-IMP and Hadacidin

Template:ABSTRACT PUBMED 16981730

About this Structure

2GCQ is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Cavitation as a mechanism of substrate discrimination by adenylosuccinate synthetases., Iancu CV, Zhou Y, Borza T, Fromm HJ, Honzatko RB, Biochemistry. 2006 Sep 26;45(38):11703-11. PMID:16981730

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