1y9g

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{{STRUCTURE_1y9g| PDB=1y9g | SCENE= }}
{{STRUCTURE_1y9g| PDB=1y9g | SCENE= }}
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'''Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose'''
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===Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose===
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==Overview==
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Exo-inulinases hydrolyze terminal, non-reducing 2,1-linked and 2,6-linked beta-d-fructofuranose residues in inulin, levan and sucrose releasing beta-d-fructose. We present the X-ray structure at 1.55A resolution of exo-inulinase from Aspergillus awamori, a member of glycoside hydrolase family 32, solved by single isomorphous replacement with the anomalous scattering method using the heavy-atom sites derived from a quick cryo-soaking technique. The tertiary structure of this enzyme folds into two domains: the N-terminal catalytic domain of an unusual five-bladed beta-propeller fold and the C-terminal domain folded into a beta-sandwich-like structure. Its structural architecture is very similar to that of another member of glycoside hydrolase family 32, invertase (beta-fructosidase) from Thermotoga maritima, determined recently by X-ray crystallography The exo-inulinase is a glycoprotein containing five N-linked oligosaccharides. Two crystal forms obtained under similar crystallization conditions differ by the degree of protein glycosylation. The X-ray structure of the enzyme:fructose complex, at a resolution of 1.87A, reveals two catalytically important residues: Asp41 and Glu241, a nucleophile and a catalytic acid/base, respectively. The distance between the side-chains of these residues is consistent with a double displacement mechanism of reaction. Asp189, which is part of the Arg-Asp-Pro motif, provides hydrogen bonds important for substrate recognition.
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{{ABSTRACT_PUBMED_15522299}}
==About this Structure==
==About this Structure==
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[[Category: Glycoside hydrolase family 32]]
[[Category: Glycoside hydrolase family 32]]
[[Category: X-ray structure]]
[[Category: X-ray structure]]
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Revision as of 21:04, 28 July 2008

Image:1y9g.png

Template:STRUCTURE 1y9g

Crystal structure of exo-inulinase from Aspergillus awamori complexed with fructose

Template:ABSTRACT PUBMED 15522299

About this Structure

1Y9G is a Single protein structure of sequence from Aspergillus awamori. Full crystallographic information is available from OCA.

Reference

Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognition., Nagem RA, Rojas AL, Golubev AM, Korneeva OS, Eneyskaya EV, Kulminskaya AA, Neustroev KN, Polikarpov I, J Mol Biol. 2004 Nov 19;344(2):471-80. PMID:15522299

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