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1njy

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[[Image:1njy.gif|left|200px]]
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{{Seed}}
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[[Image:1njy.png|left|200px]]
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{{STRUCTURE_1njy| PDB=1njy | SCENE= }}
{{STRUCTURE_1njy| PDB=1njy | SCENE= }}
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'''THYMINE-THYMINE MISMATCH AT THE POLYMERASE ACTIVE SITE'''
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===THYMINE-THYMINE MISMATCH AT THE POLYMERASE ACTIVE SITE===
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==Overview==
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Accurate DNA replication is essential for genomic stability. One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a "short-term memory" of replication errors, responding to mismatches up to four base pairs in from the primer terminus. Here we a present a structural characterization of all 12 possible mismatches captured at the growing primer terminus in the active site of a polymerase. Our observations suggest four mechanisms that lead to mismatch-induced stalling of the polymerase. Furthermore, we have observed the effects of extending a mismatch up to six base pairs from the primer terminus and find that long-range distortions in the DNA transmit the presence of the mismatch back to the enzyme active site, suggesting the structural basis for the short-term memory of replication errors.
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The line below this paragraph, {{ABSTRACT_PUBMED_15035983}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15035983 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15035983}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15035983 15035983]
Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15035983 15035983]
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Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations., Johnson SJ, Taylor JS, Beese LS, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3895-900. Epub 2003 Mar 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12649320 12649320]
[[Category: DNA-directed DNA polymerase]]
[[Category: DNA-directed DNA polymerase]]
[[Category: Beese, L S.]]
[[Category: Beese, L S.]]
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[[Category: Klenow fragment]]
[[Category: Klenow fragment]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:37:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:04:16 2008''

Revision as of 21:04, 28 July 2008

Image:1njy.png

Template:STRUCTURE 1njy

THYMINE-THYMINE MISMATCH AT THE POLYMERASE ACTIVE SITE

Template:ABSTRACT PUBMED 15035983

About this Structure

Full crystallographic information is available from OCA.

Reference

Structures of mismatch replication errors observed in a DNA polymerase., Johnson SJ, Beese LS, Cell. 2004 Mar 19;116(6):803-16. PMID:15035983

Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations., Johnson SJ, Taylor JS, Beese LS, Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3895-900. Epub 2003 Mar 20. PMID:12649320

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