2hjn

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{{STRUCTURE_2hjn| PDB=2hjn | SCENE= }}
{{STRUCTURE_2hjn| PDB=2hjn | SCENE= }}
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'''Structural and functional analysis of Saccharomyces cerevisiae Mob1'''
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===Structural and functional analysis of Saccharomyces cerevisiae Mob1===
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==Overview==
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The Mob proteins function as activator subunits for the Dbf2/Dbf20 family of protein kinases. Human and Xenopus Mob1 protein structures corresponding to the most conserved C-terminal core, but lacking the variable N-terminal region, have been reported and provide a framework for understanding the mechanism of Dbf2/Dbf20 regulation. Here, we report the 2.0 A X-ray crystal structure of Saccharomyces cerevisiae Mob1 containing both the conserved C-terminal core and the variable N-terminal region. Within the N-terminal region, three novel structural elements are observed; namely, an alpha-helix denoted H0, a strand-like element denoted S0 and a short beta strand denoted S-1. Helix H0 associates in an intermolecular manner with a second Mob1 molecule to form a Mob1 homodimer. Strand S0 binds to the core domain in an intramolecular manner across a putative Dbf2 binding site mapped by Mob1 temperature-sensitive alleles and NMR binding experiments. In vivo functional analysis demonstrates that Mob1 mutants that target helix H0 or its reciprocal binding site are biologically compromised. The N-terminal region of Mob1 thus contains structural elements that are functionally important.
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The line below this paragraph, {{ABSTRACT_PUBMED_16934835}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16934835 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16934835}}
==About this Structure==
==About this Structure==
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[[Category: Sicheri, F.]]
[[Category: Sicheri, F.]]
[[Category: Homodimer]]
[[Category: Homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:22:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:08:40 2008''

Revision as of 21:08, 28 July 2008

Image:2hjn.png

Template:STRUCTURE 2hjn

Structural and functional analysis of Saccharomyces cerevisiae Mob1

Template:ABSTRACT PUBMED 16934835

About this Structure

2HJN is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of Saccharomyces cerevisiae Mob1., Mrkobrada S, Boucher L, Ceccarelli DF, Tyers M, Sicheri F, J Mol Biol. 2006 Sep 22;362(3):430-40. Epub 2006 Aug 24. PMID:16934835

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