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1se9

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[[Image:1se9.gif|left|200px]]
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{{STRUCTURE_1se9| PDB=1se9 | SCENE= }}
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'''Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana'''
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===Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana===
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==Overview==
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Structural proteomics requires robust, scalable methods. Here we describe a wheat germ cell-free platform for protein production that supports efficient NMR structural studies of eukaryotic proteins and offers advantages over cell-based methods. To illustrate this platform, we describe its application to a specific target (At3g01050.1) from Arabidopsis thaliana. After cloning the target gene into a specialized plasmid, we carry out a small-scale (50 mul) in vitro sequential transcription and translation trial to ascertain the level of protein production and solubility. Next, we prepare mRNA for use in a 4-ml semicontinuous cell-free translation reaction to incorporate (15)N-labeled amino acids into a protein sample that we purify and test for suitability for NMR structural analysis. We then repeat the cell-free approach with (13)C,(15)N-labeled amino acids to prepare a doubly labeled sample. The three-dimensional (3D) structure of At3g01050.1 shows that this protein is an unusual member of the beta-grasp protein family.
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(as it appears on PubMed at http://www.pubmed.gov), where 15782178 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15782178}}
==About this Structure==
==About this Structure==
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1SE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA].
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1SE9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE9 OCA].
==Reference==
==Reference==
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[[Category: Ubiquitin-like]]
[[Category: Ubiquitin-like]]
[[Category: Wheat germ]]
[[Category: Wheat germ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:21:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:10:59 2008''

Revision as of 21:11, 28 July 2008

Image:1se9.png

Template:STRUCTURE 1se9

Structure of At3g01050, a ubiquitin-fold protein from Arabidopsis thaliana

Template:ABSTRACT PUBMED 15782178

About this Structure

1SE9 is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

Cell-free protein production and labeling protocol for NMR-based structural proteomics., Vinarov DA, Lytle BL, Peterson FC, Tyler EM, Volkman BF, Markley JL, Nat Methods. 2004 Nov;1(2):149-53. Epub 2004 Oct 21. PMID:15782178

Page seeded by OCA on Tue Jul 29 00:10:59 2008

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