2fk4

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{{STRUCTURE_2fk4| PDB=2fk4 | SCENE= }}
{{STRUCTURE_2fk4| PDB=2fk4 | SCENE= }}
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'''Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein'''
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===Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein===
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==Overview==
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Oncoprotein E6 is essential for oncogenesis induced by human papillomaviruses (HPVs). The solution structure of HPV16-E6 C-terminal domain reveals a zinc binding fold. A model of full-length E6 is proposed and analyzed in the context of HPV evolution. E6 appears as a chameleon protein combining a conserved structural scaffold with highly variable surfaces participating in generic or specialized HPV functions. We investigated surface residues involved in two specialized activities of high-risk genital HPV E6: p53 tumor suppressor degradation and nucleic acid binding. Screening of E6 surface mutants identified an in vivo p53 degradation-defective mutant that fails to recruit p53 to ubiquitin ligase E6AP and restores high p53 levels in cervical carcinoma cells by competing with endogeneous E6. We also mapped the nucleic acid binding surface of E6, the positive potential of which correlates with genital oncogenicity. E6 structure-function analysis provides new clues for understanding and counteracting the complex pathways of HPV-mediated pathogenesis.
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The line below this paragraph, {{ABSTRACT_PUBMED_16507364}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16507364 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16507364}}
==About this Structure==
==About this Structure==
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2FK4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK4 OCA].
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2FK4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_16 Human papillomavirus type 16]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FK4 OCA].
==Reference==
==Reference==
Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16507364 16507364]
Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16507364 16507364]
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1H and 15N resonance assignment, secondary structure and dynamic behaviour of the C-terminal domain of human papillomavirus oncoprotein E6., Nomine Y, Charbonnier S, Miguet L, Potier N, Van Dorsselaer A, Atkinson RA, Trave G, Kieffer B, J Biomol NMR. 2005 Feb;31(2):129-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15772752 15772752]
[[Category: Human papillomavirus type 16]]
[[Category: Human papillomavirus type 16]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Oncoprotein]]
[[Category: Oncoprotein]]
[[Category: Zinc binding domain]]
[[Category: Zinc binding domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:59:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:14:31 2008''

Revision as of 21:14, 28 July 2008

Template:STRUCTURE 2fk4

Solution structure of the C-terminal zinc binding domain of the HPV16 E6 oncoprotein

Template:ABSTRACT PUBMED 16507364

About this Structure

2FK4 is a Single protein structure of sequence from Human papillomavirus type 16. Full experimental information is available from OCA.

Reference

Structural and functional analysis of E6 oncoprotein: insights in the molecular pathways of human papillomavirus-mediated pathogenesis., Nomine Y, Masson M, Charbonnier S, Zanier K, Ristriani T, Deryckere F, Sibler AP, Desplancq D, Atkinson RA, Weiss E, Orfanoudakis G, Kieffer B, Trave G, Mol Cell. 2006 Mar 3;21(5):665-78. PMID:16507364

1H and 15N resonance assignment, secondary structure and dynamic behaviour of the C-terminal domain of human papillomavirus oncoprotein E6., Nomine Y, Charbonnier S, Miguet L, Potier N, Van Dorsselaer A, Atkinson RA, Trave G, Kieffer B, J Biomol NMR. 2005 Feb;31(2):129-41. PMID:15772752

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