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2opo

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{{Seed}}
[[Image:2opo.gif|left|200px]]
[[Image:2opo.gif|left|200px]]
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{{STRUCTURE_2opo| PDB=2opo | SCENE= }}
{{STRUCTURE_2opo| PDB=2opo | SCENE= }}
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'''Crystal structure of the calcium-binding pollen allergen Che a 3'''
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===Crystal structure of the calcium-binding pollen allergen Che a 3===
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==Overview==
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Two EF-hand calcium-binding allergens (polcalcins) occur in the pollen of a wide variety of unrelated plants as highly cross-reactive allergenic molecules. We report the expression, purification, immunological characterization, and the 1.75-A crystal structure of recombinant Che a 3 (rChe a 3), the polcalcin from the weed Chenopodium album. The three-dimensional structure of rChe a 3 resembles an alpha-helical fold that is essentially identical with that of the two EF-hand allergens from birch pollen, Bet v 4, and timothy grass pollen, Phl p 7. The extensive cross-reactivity between Che a 3 and Phl p 7 is demonstrated by competition experiments with IgE Abs from allergic patients as well as specific Ab probes. Amino acid residues that are conserved for the two EF-hand allergen family were identified in multiple sequence alignments of polcalcins from 15 different plants. Next, the three-dimensional structures of rChe a 3, rPhl p 7, and rBet v 4 were used to identify conserved amino acids with high surface exposition to visualize surface patches as potential targets for the polyclonal IgE Ab response of allergic patients. The essentially identical three-dimensional structures of rChe a 3, rPhl p 7, and rBet v 4 explain the extensive cross-reactivity of allergic patients IgE Abs with two EF-hand allergens from unrelated plants. In addition, analyzing the three-dimensional structures of cross-reactive Ags for conserved and surface exposed amino acids may be a first approach to mapping the conformational epitopes on disease-related Ags that are recognized by polyclonal patient Abs.
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The line below this paragraph, {{ABSTRACT_PUBMED_18250440}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18250440 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18250440}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens., Verdino P, Barderas R, Villalba M, Westritschnig K, Valenta R, Rodriguez R, Keller W, J Immunol. 2008 Feb 15;180(4):2313-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18250440 18250440]
Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens., Verdino P, Barderas R, Villalba M, Westritschnig K, Valenta R, Rodriguez R, Keller W, J Immunol. 2008 Feb 15;180(4):2313-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18250440 18250440]
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Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: isolation, amino acid sequences, and immunologic properties., Barderas R, Villalba M, Pascual CY, Batanero E, Rodriguez R, J Allergy Clin Immunol. 2004 Jun;113(6):1192-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15208604 15208604]
[[Category: Chenopodium album]]
[[Category: Chenopodium album]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Domain-swapping]]
[[Category: Domain-swapping]]
[[Category: Ef-hand]]
[[Category: Ef-hand]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 9 14:40:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:18:17 2008''

Revision as of 21:18, 28 July 2008

Image:2opo.gif

Template:STRUCTURE 2opo

Crystal structure of the calcium-binding pollen allergen Che a 3

Template:ABSTRACT PUBMED 18250440

About this Structure

2OPO is a Single protein structure of sequence from Chenopodium album. This structure supersedes the now removed PDB entry 1pmz. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of the cross-reactive pollen allergen Che a 3: visualizing cross-reactivity on the molecular surfaces of weed, grass, and tree pollen allergens., Verdino P, Barderas R, Villalba M, Westritschnig K, Valenta R, Rodriguez R, Keller W, J Immunol. 2008 Feb 15;180(4):2313-21. PMID:18250440

Profilin (Che a 2) and polcalcin (Che a 3) are relevant allergens of Chenopodium album pollen: isolation, amino acid sequences, and immunologic properties., Barderas R, Villalba M, Pascual CY, Batanero E, Rodriguez R, J Allergy Clin Immunol. 2004 Jun;113(6):1192-8. PMID:15208604

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