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1npn

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{{STRUCTURE_1npn| PDB=1npn | SCENE= }}
{{STRUCTURE_1npn| PDB=1npn | SCENE= }}
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'''Crystal structure of a copper reconstituted H145A mutant of nitrite reductase from Alcaligenes faecalis'''
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===Crystal structure of a copper reconstituted H145A mutant of nitrite reductase from Alcaligenes faecalis===
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==Overview==
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Variants of the copper-containing nitrite reductase (NiR) of Alcaligenes faecalis S6 were constructed by site-directed mutagenesis, by which the C-terminal histidine ligand (His145) of the Cu in the type-1 site was replaced by an alanine or a glycine. The type-1 sites in the NiR variants as isolated, are in the reduced form, but can be oxidized in the presence of external ligands, like (substituted) imidazoles and chloride. The reduction potential of the type-1 site of NiR-H145A reconstituted with imidazole amounts to 505 mV vs NHE (20 degrees C, pH 7, 10 mM imidazole), while for the native type-1 site it amounts to 260 mV. XRD data on crystals of the reduced and oxidized NiR-H145A variant show that in the reduced type-1 site the metal is 3-coordinated, but in the oxidized form takes up a ligand from the solution. With the fourth (exogenous) ligand in place the type-1 site is able to accept electrons at about the same rate as the wt NiR, but it is unable to pass the electron onto the type-2 site, leading to loss of enzymatic activity. It is argued that the uptake of an electron by the mutated type-1 site is accompanied by a loss of the exogenous ligand and a concomitant rise of the redox potential. This rise effectively traps the electron in the type-1 site.
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==About this Structure==
==About this Structure==
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[[Category: Wijma, H J.]]
[[Category: Wijma, H J.]]
[[Category: Copper nitrite reductase]]
[[Category: Copper nitrite reductase]]
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Revision as of 21:18, 28 July 2008

Image:1npn.png

Template:STRUCTURE 1npn

Crystal structure of a copper reconstituted H145A mutant of nitrite reductase from Alcaligenes faecalis

Template:ABSTRACT PUBMED 12680761

About this Structure

1NPN is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

Reference

Reconstitution of the type-1 active site of the H145G/A variants of nitrite reductase by ligand insertion., Wijma HJ, Boulanger MJ, Molon A, Fittipaldi M, Huber M, Murphy ME, Verbeet MP, Canters GW, Biochemistry. 2003 Apr 15;42(14):4075-83. PMID:12680761

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