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1omz
From Proteopedia
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(New page: 200px<br /><applet load="1omz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1omz, resolution 2.10Å" /> '''crystal structure of...)
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Revision as of 20:54, 20 November 2007
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crystal structure of mouse alpha-1,4-N-acetylhexosaminyltransferase (EXTL2) in complex with UDPGalNAc
Overview
EXTL2, an alpha1,4-N-acetylhexosaminyltransferase, catalyzes the transfer, reaction of N-acetylglucosamine and N-acetylgalactosamine from the, respective UDP-sugars to the non-reducing end of [glucuronic, acid]beta1-3[galactose]beta1-O-naphthalenemethanol, an acceptor substrate, analog of the natural common linker of various glycosylaminoglycans. We, have solved the x-ray crystal structure of the catalytic domain of mouse, EXTL2 in the apo-form and with donor substrates UDP-N-acetylglucosamine, and UDP-N-acetylgalactosamine. In addition, a structure of the ternary, complex with UDP and the acceptor substrate analog [glucuronic, acid]beta1-3[galactose]beta1-O-naphthalenemethanol has been determined., These structures reveal three highly conserved residues, Asn-243, Asp-246, and Arg-293, located at the active site. Mutation of these residues, greatly decreases the activity. In the ternary complex, an interaction, exists between the beta-phosphate of the UDP leaving group and the, acceptor hydroxyl of the substrate that may play a functional role in, catalysis. These structures represent the first structures from the, exostosin gene family and provide important insight into the mechanisms of, alpha1,4-N-acetylhexosaminyl transfer in heparan biosynthesis.
About this Structure
1OMZ is a Single protein structure of sequence from Mus musculus with MN, UD2 and EDO as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of an alpha 1,4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis., Pedersen LC, Dong J, Taniguchi F, Kitagawa H, Krahn JM, Pedersen LG, Sugahara K, Negishi M, J Biol Chem. 2003 Apr 18;278(16):14420-8. Epub 2003 Jan 31. PMID:12562774
Page seeded by OCA on Tue Nov 20 23:01:19 2007
Categories: Mus musculus | Single protein | Dong, J. | Kitagawa, H. | Krahn, J.M. | Negishi, M. | Pedersen, L.C. | Pedersen, L.G. | Sugahara, K. | Taniguchi, F. | EDO | MN | UD2 | Dxd motif | Rossmann fold
