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2bwh

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[[Image:2bwh.gif|left|200px]]
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{{STRUCTURE_2bwh| PDB=2bwh | SCENE= }}
{{STRUCTURE_2bwh| PDB=2bwh | SCENE= }}
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'''LAUE STRUCTURE OF A SHORT LIVED STATE OF L29W MYOGLOBIN'''
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===LAUE STRUCTURE OF A SHORT LIVED STATE OF L29W MYOGLOBIN===
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==Overview==
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By using time-resolved x-ray crystallography at room temperature, structural relaxations and ligand migration were examined in myoglobin (Mb) mutant L29W from nanoseconds to seconds after photodissociation of carbon monoxide (CO) from the heme iron by nanosecond laser pulses. The data were analyzed in terms of transient kinetics by fitting trial functions to integrated difference electron density values obtained from select structural moieties, thus allowing a quantitative description of the processes involved. The observed relaxations are linked to other investigations on protein dynamics. At the earliest times, the heme has already completely relaxed into its domed deoxy structure, and there is no photo-dissociated CO visible at the primary docking site. Initial relaxations of larger globin moieties are completed within several hundred nanoseconds. They influence the concomitant migration of photo-dissociated CO to the Xe1 site, where it appears at approximately 300 ns and leaves again at approximately 1.5 ms. The extremely long residence time in Xe1 as compared with wild-type MbCO implies that, in the latter protein, the CO exits the protein from Xe1 predominantly via the distal pocket. A well-defined deligated state is populated between approximately 2 micros and approximately 1 ms; its structure is very similar to the equilibrium deoxy structure. Between 1.5 and 20 ms, no CO is visible in the protein interior; it is either distributed among many sites within the protein or has escaped to the solvent. Finally, recombination at the heme iron occurs after &gt;20 ms.
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==About this Structure==
==About this Structure==
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[[Category: Oxygen transport]]
[[Category: Oxygen transport]]
[[Category: Time-resolved x-ray structure determination]]
[[Category: Time-resolved x-ray structure determination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:54:00 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:26:41 2008''

Revision as of 21:26, 28 July 2008

Image:2bwh.png

Template:STRUCTURE 2bwh

LAUE STRUCTURE OF A SHORT LIVED STATE OF L29W MYOGLOBIN

Template:ABSTRACT PUBMED 16085709

About this Structure

2BWH is a Single protein structure. Full crystallographic information is available from OCA.

Reference

Ligand migration pathway and protein dynamics in myoglobin: a time-resolved crystallographic study on L29W MbCO., Schmidt M, Nienhaus K, Pahl R, Krasselt A, Anderson S, Parak F, Nienhaus GU, Srajer V, Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11704-9. Epub 2005 Aug 5. PMID:16085709

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