1os8

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[[Image:1os8.jpg|left|200px]]
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{{STRUCTURE_1os8| PDB=1os8 | SCENE= }}
{{STRUCTURE_1os8| PDB=1os8 | SCENE= }}
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'''RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN'''
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===RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN===
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==Overview==
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Streptomyces griseus trypsin (SGT) was chosen as a model scaffold for the development of serine proteases with enhanced substrate specificity. Recombinant SGT has been produced in a Bacillus subtilis expression system in a soluble active form and purified to homogeneity. The recombinant and native proteases have nearly identical enzymatic properties and structures. Four SGT mutants with alterations in the S1 substrate binding pocket (T190A, T190P, T190S, and T190V) were also expressed. The T190P mutant demonstrated the largest shift to a preference for Arg versus Lys in the P1 site. This was shown by a minor reduction in catalytic activity toward an Arg-containing substrate (k(cat) reduction of 25%). The crystal structures of the recombinant SGT and the T190P mutant in a complex with the inhibitor benzamidine were obtained at high resolution (approximately 1.9 A). The increase in P1 specificity, achieved with minimal effect on the catalytic efficiency, demonstrates that the T190P mutant is an ideal candidate for the design of additional substrate specificity engineered into the S2 to S4 binding pockets.
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(as it appears on PubMed at http://www.pubmed.gov), where 12885239 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12885239}}
==About this Structure==
==About this Structure==
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[[Category: Serine protease]]
[[Category: Serine protease]]
[[Category: Trypsin]]
[[Category: Trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:13:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:29:07 2008''

Revision as of 21:29, 28 July 2008

Image:1os8.png

Template:STRUCTURE 1os8

RECOMBINANT STREPTOMYCES GRISEUS TRYPSIN

Template:ABSTRACT PUBMED 12885239

About this Structure

1OS8 is a Single protein structure of sequence from Streptomyces griseus. Full crystallographic information is available from OCA.

Reference

Engineering the primary substrate specificity of Streptomyces griseus trypsin., Page MJ, Wong SL, Hewitt J, Strynadka NC, MacGillivray RT, Biochemistry. 2003 Aug 5;42(30):9060-6. PMID:12885239

Page seeded by OCA on Tue Jul 29 00:29:07 2008

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