1zof

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{{STRUCTURE_1zof| PDB=1zof | SCENE= }}
{{STRUCTURE_1zof| PDB=1zof | SCENE= }}
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'''Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter Pylori'''
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===Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter Pylori===
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==Overview==
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The AhpC protein from H. pylori, a thioredoxin (Trx)-dependent alkyl hydroperoxide-reductase, is a member of the ubiquitous 2-Cys peroxiredoxins family (2-Cys Prxs), a group of thiol-specific antioxidant enzymes. Prxs exert the protective antioxidant role in cells through their peroxidase activity, whereby hydrogen peroxide, peroxynitrite and a wide range of organic hydroperoxides (ROOH) are reduced and detoxified (ROOH + 2e(-)--&gt;ROH + H2O). In this study AhpC has been cloned and overexpressed in E. coli. After purification to homogeneity, crystals of the recombinant protein were grown. They diffract to 2.95 A resolution using synchrotron radiation. The crystal structure of AhpC has been determined using the molecular replacement method (R = 23.6%, R(free) = 25.9%). The model, similar in the overall to other members of the 2-Cys Prx family crystallized as toroide-shaped complexes, consists of a pentameric arrangement of homodimers [(alpha2)5 decamer]. The model of AhpC from H. pylori presents significant differences with respect to other members of the family: apart from some loop regions, alpha5-helix and the C-terminus is shifted, preventing the C-terminal tail of the second subunit from extending toward this region of the molecule. Oligomerization properties of AhpC have been also characterized by gel filtration chromatography.
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(as it appears on PubMed at http://www.pubmed.gov), where 16213196 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16213196}}
==About this Structure==
==About this Structure==
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[[Category: Decamer]]
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[[Category: Toroide-shaped complex]]
[[Category: Toroide-shaped complex]]
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Revision as of 21:31, 28 July 2008

Image:1zof.png

Template:STRUCTURE 1zof

Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter Pylori

Template:ABSTRACT PUBMED 16213196

About this Structure

1ZOF is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.

Reference

Crystal structure of alkyl hydroperoxide-reductase (AhpC) from Helicobacter pylori., Papinutto E, Windle HJ, Cendron L, Battistutta R, Kelleher D, Zanotti G, Biochim Biophys Acta. 2005 Dec 1;1753(2):240-6. Epub 2005 Sep 21. PMID:16213196

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