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2omz

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[[Image:2omz.gif|left|200px]]
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{{STRUCTURE_2omz| PDB=2omz | SCENE= }}
{{STRUCTURE_2omz| PDB=2omz | SCENE= }}
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'''Crystal structure of InlA Y369A/hEC1 complex'''
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===Crystal structure of InlA Y369A/hEC1 complex===
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==Overview==
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Biological processes essentially all depend on the specific recognition between macromolecules and their interaction partners. Although many such interactions have been characterized both structurally and biophysically, the thermodynamic effects of small atomic changes remain poorly understood. Based on the crystal structure of the bacterial invasion protein internalin (InlA) of Listeria monocytogenes in complex with its human receptor E-cadherin (hEC1), we analyzed the interface to identify single amino acid substitutions in InlA that would potentially improve the overall quality of interaction and hence increase the weak binding affinity of the complex. Dissociation constants of InlA-variant/hEC1 complexes, as well as enthalpy and entropy of binding, were quantified by isothermal titration calorimetry. All single substitutions indeed significantly increase binding affinity. Structural changes were verified crystallographically at &lt; or =2.0-A resolution, allowing thermodynamic characteristics of single substitutions to be rationalized structurally and providing unique insights into atomic contributions to binding enthalpy and entropy. Structural and thermodynamic data of all combinations of individual substitutions result in a thermodynamic network, allowing the source of cooperativity between distant recognition sites to be identified. One such pair of single substitutions improves affinity 5,000-fold. We thus demonstrate that rational reengineering of protein complexes is possible by making use of physically distant hot spots of recognition.
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(as it appears on PubMed at http://www.pubmed.gov), where 17715295 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17715295}}
==About this Structure==
==About this Structure==
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[[Category: Leucine-rich-repeat]]
[[Category: Leucine-rich-repeat]]
[[Category: Nvasion protein]]
[[Category: Nvasion protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:14:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:36:05 2008''

Revision as of 21:36, 28 July 2008

Image:2omz.png

Template:STRUCTURE 2omz

Crystal structure of InlA Y369A/hEC1 complex

Template:ABSTRACT PUBMED 17715295

About this Structure

2OMZ is a Protein complex structure of sequences from Homo sapiens and Listeria monocytogenes. Full crystallographic information is available from OCA.

Reference

Thermodynamically reengineering the listerial invasion complex InlA/E-cadherin., Wollert T, Heinz DW, Schubert WD, Proc Natl Acad Sci U S A. 2007 Aug 28;104(35):13960-5. Epub 2007 Aug 22. PMID:17715295

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