2z8s

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{{STRUCTURE_2z8s| PDB=2z8s | SCENE= }}
{{STRUCTURE_2z8s| PDB=2z8s | SCENE= }}
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'''Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid'''
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===Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid===
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==Overview==
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Rhamnogalacturonan (RG) lyase produced by plant pathogenic and saprophytic microbes plays an important role in degrading plant cell walls. An extracellular RG lyase YesW from saprophytic Bacillus subtilis is a member of polysaccharide lyase family 11 and cleaves glycoside bonds in polygalacturonan as well as RG type-I through a beta-elimination reaction. Crystal structures of YesW and its complex with galacturonan disaccharide, a reaction product analogue, were determined at 1.4 and 2.5 A resolutions with final R-factors of 16.4% and 16.6%, respectively. The enzyme is composed of an eight-bladed beta-propeller with a deep cleft in the center as a basic scaffold, and its structural fold has not been seen in polysaccharide lyases analyzed thus far. Structural analysis of the disaccharide-bound YesW and a site-directed mutagenesis study suggested that Arg-452 and Lys-535 stabilize the carboxyl group of the acidic polysaccharide molecule and Tyr-595 makes a stack interaction with the sugar pyranose ring. In addition to amino acid residues binding to the disaccharide, one calcium ion, which is coordinated by Asp-401, Glu-422, His-363, and His-399, may mediate the enzyme activity. This is, to our knowledge, the first report of a new structural category with a beta-propeller fold in polysaccharide lyases and provides structural insights into substrate binding by RG lyase.
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(as it appears on PubMed at http://www.pubmed.gov), where 17947240 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17947240}}
==About this Structure==
==About this Structure==
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[[Category: Beta-propeller]]
[[Category: Beta-propeller]]
[[Category: Lyase]]
[[Category: Lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:02:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:36:41 2008''

Revision as of 21:36, 28 July 2008

Image:2z8s.png

Template:STRUCTURE 2z8s

Crystal structure of rhamnogalacturonan lyase YesW complexed with digalacturonic acid

Template:ABSTRACT PUBMED 17947240

About this Structure

2Z8S is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

A novel structural fold in polysaccharide lyases: Bacillus subtilis family 11 rhamnogalacturonan lyase YesW with an eight-bladed beta-propeller., Ochiai A, Itoh T, Maruyama Y, Kawamata A, Mikami B, Hashimoto W, Murata K, J Biol Chem. 2007 Dec 21;282(51):37134-45. Epub 2007 Oct 17. PMID:17947240

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