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2q91

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[[Image:2q91.jpg|left|200px]]
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{{STRUCTURE_2q91| PDB=2q91 | SCENE= }}
{{STRUCTURE_2q91| PDB=2q91 | SCENE= }}
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'''Structure of the Ca2+-Bound Activated Form of the S100A4 Metastasis Factor'''
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===Structure of the Ca2+-Bound Activated Form of the S100A4 Metastasis Factor===
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==Overview==
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S100A4, also known as mts1, is a member of the S100 family of Ca (2+)-binding proteins that is directly involved in tumor invasion and metastasis via interactions with specific protein targets, including nonmuscle myosin-IIA (MIIA). Human S100A4 binds two Ca (2+) ions with the typical EF-hand exhibiting an affinity that is nearly 1 order of magnitude tighter than that of the pseudo-EF-hand. To examine how Ca (2+) modifies the overall organization and structure of the protein, we determined the 1.7 A crystal structure of the human Ca (2+)-S100A4. Ca (2+) binding induces a large reorientation of helix 3 in the typical EF-hand. This reorganization exposes a hydrophobic cleft that is comprised of residues from the hinge region, helix 3, and helix 4, which afford specific target recognition and binding. The Ca (2+)-dependent conformational change is required for S100A4 to bind peptide sequences derived from the C-terminal portion of the MIIA rod with submicromolar affinity. In addition, the level of binding of Ca (2+) to both EF-hands increases by 1 order of magnitude in the presence of MIIA. NMR spectroscopy studies demonstrate that following titration with a MIIA peptide, the largest chemical shift perturbations and exchange broadening effects occur for residues in the hydrophobic pocket of Ca (2+)-S100A4. Most of these residues are not exposed in apo-S100A4 and explain the Ca (2+) dependence of formation of the S100A4-MIIA complex. These studies provide the foundation for understanding S100A4 target recognition and may support the development of reagents that interfere with S100A4 function.
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(as it appears on PubMed at http://www.pubmed.gov), where 18410126 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18410126}}
==About this Structure==
==About this Structure==
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[[Category: Myosin]]
[[Category: Myosin]]
[[Category: S100a4]]
[[Category: S100a4]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 7 08:50:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:43:36 2008''

Revision as of 21:43, 28 July 2008

Image:2q91.png

Template:STRUCTURE 2q91

Structure of the Ca2+-Bound Activated Form of the S100A4 Metastasis Factor

Template:ABSTRACT PUBMED 18410126

About this Structure

2Q91 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of Ca(2+)-Bound S100A4 and Its Interaction with Peptides Derived from Nonmuscle Myosin-IIA., Malashkevich VN, Varney KM, Garrett SC, Wilder PT, Knight D, Charpentier TH, Ramagopal UA, Almo SC, Weber DJ, Bresnick AR, Biochemistry. 2008 May 6;47(18):5111-5126. Epub 2008 Apr 15. PMID:18410126

Page seeded by OCA on Tue Jul 29 00:43:36 2008

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