1z69

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[[Image:1z69.gif|left|200px]]
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{{STRUCTURE_1z69| PDB=1z69 | SCENE= }}
{{STRUCTURE_1z69| PDB=1z69 | SCENE= }}
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'''Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420'''
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===Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420===
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==Overview==
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Methylenetetratetrahydromethanopterin reductase (Mer) is involved in CO(2) reduction to methane in methanogenic archaea and catalyses the reversible reduction of methylenetetrahydromethanopterin (methylene-H(4)MPT) to methyl-H(4)MPT with coenzyme F(420)H(2), which is a reduced 5'-deazaflavin. Mer was recently established as a TIM barrel structure containing a nonprolyl cis-peptide bond but the binding site of the substrates remained elusive. We report here on the crystal structure of Mer in complex with F(420) at 2.6 A resolution. The isoalloxazine ring is present in a pronounced butterfly conformation, being induced from the Re-face of F(420) by a bulge that contains the non-prolyl cis-peptide bond. The bindingmode of F(420) is very similar to that in F(420)-dependent alcohol dehydrogenase Adf despite the low sequence identity of 21%. Moreover, binding of F(420) to the apoenzyme was only associated with minor conformational changes of the polypeptide chain. These findings allowed us to build an improved model of FMN into its binding site in bacterial luciferase, which belongs to the same structural family as Mer and Adf and also contains a nonprolyl cis-peptide bond in an equivalent position.
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(as it appears on PubMed at http://www.pubmed.gov), where 15937276 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15937276}}
==About this Structure==
==About this Structure==
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[[Category: Thauer, R K.]]
[[Category: Thauer, R K.]]
[[Category: Warkentin, E.]]
[[Category: Warkentin, E.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:13:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:49:33 2008''

Revision as of 21:49, 28 July 2008

Image:1z69.png

Template:STRUCTURE 1z69

Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420

Template:ABSTRACT PUBMED 15937276

About this Structure

1Z69 is a Single protein structure of sequence from Methanosarcina barkeri. Full crystallographic information is available from OCA.

Reference

Crystal structure of methylenetetrahydromethanopterin reductase (Mer) in complex with coenzyme F420: Architecture of the F420/FMN binding site of enzymes within the nonprolyl cis-peptide containing bacterial luciferase family., Aufhammer SW, Warkentin E, Ermler U, Hagemeier CH, Thauer RK, Shima S, Protein Sci. 2005 Jul;14(7):1840-9. Epub 2005 Jun 3. PMID:15937276

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