2fv1

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[[Image:2fv1.gif|left|200px]]
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{{Seed}}
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[[Image:2fv1.png|left|200px]]
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{{STRUCTURE_2fv1| PDB=2fv1 | SCENE= }}
{{STRUCTURE_2fv1| PDB=2fv1 | SCENE= }}
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'''UGL_D88N/dGlcA-GlcNAc'''
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===UGL_D88N/dGlcA-GlcNAc===
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==Overview==
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Bacterial unsaturated glucuronyl hydrolases (UGLs) together with polysaccharide lyases are responsible for the complete depolymerization of mammalian extracellular matrix glycosaminoglycans. UGL acts on various oligosaccharides containing unsaturated glucuronic acid (DeltaGlcA) at the nonreducing terminus and releases DeltaGlcA through hydrolysis. In this study, we demonstrate the substrate recognition mechanism of the UGL of Bacillus sp. GL1 by determining the X-ray crystallographic structure of its substrate-enzyme complexes. The tetrasaccharide-enzyme complex demonstrated that at least four subsites are present in the active pocket. Although several amino acid residues are crucial for substrate binding, the enzyme strongly recognizes DeltaGlcA at subsite -1 through the formation of hydrogen bonds and stacking interactions, and prefers N-acetyl-d-galactosamine and glucose rather than N-acetyl-d-glucosamine as a residue accommodated in subsite +1, due to the steric hindrance.
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The line below this paragraph, {{ABSTRACT_PUBMED_16630576}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16630576 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16630576}}
==About this Structure==
==About this Structure==
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[[Category: Murata, K.]]
[[Category: Murata, K.]]
[[Category: Alpha6/alpha6-barrel]]
[[Category: Alpha6/alpha6-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:20:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:51:59 2008''

Revision as of 21:52, 28 July 2008

Image:2fv1.png

Template:STRUCTURE 2fv1

UGL_D88N/dGlcA-GlcNAc

Template:ABSTRACT PUBMED 16630576

About this Structure

2FV1 is a Single protein structure of sequence from Bacillus sp.. Full crystallographic information is available from OCA.

Reference

Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1., Itoh T, Hashimoto W, Mikami B, Murata K, Biochem Biophys Res Commun. 2006 May 26;344(1):253-62. PMID:16630576

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